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Titolo:
Morphometric analysis of erythrocyte aggregates in vitro: Pharmacological application with Bunflomedil (R)
Autore:
Carpentier, PH; Imbert, B; Picart, C; Brasseur, S; Malterre, C;
Indirizzi:
Univ Grenoble 1, Lab Rheol, UMR CNRS, F-38041 Grenoble, France Univ Grenoble 1 Grenoble France F-38041 R CNRS, F-38041 Grenoble, France
Titolo Testata:
JOURNAL DES MALADIES VASCULAIRES
fascicolo: 5, volume: 25, anno: 2000,
pagine: 356 - 359
SICI:
0398-0499(200012)25:5<356:MAOEAI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
FRE
Soggetto:
BUFLOMEDIL; VASOMOTION;
Keywords:
hemorheology; red blood cell aggregation; digital image analysis; pharmacology; Buflomedil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Carpentier, PH CHU Grenoble, Dept Pluridisciplinaire Med, Unite Med Vasc, F-38043 Grenoble, France CHU Grenoble Grenoble France F-38043 043 Grenoble, France
Citazione:
P.H. Carpentier et al., "Morphometric analysis of erythrocyte aggregates in vitro: Pharmacological application with Bunflomedil (R)", J MAL VASC, 25(5), 2000, pp. 356-359

Abstract

Erythrocyte aggregation is usually evaluated through indirect measurementssuch as the sedimentation rate and rheometric measurements of aggregation and disaggregation thresholds. The aim of this study was a preliminary evaluation of a morphometric approach of aggregates in vitro. A sample of blood (on EDTA) was examined under the microscope, and a picture with magnification x 640 was obtained after stabilization of the cellular structure. The digital image was analyzed with a home made dedicated software that allows the measurement of the cellular density and the evaluationof several shape parameters. Ten samples obtained from different donors were analysed within one hour after blood withdrawal and 24 hours later (preservation temperature: 4 degreesC), with and without adjunction of Buflomedil(R) (4 mug/ml). Results showed an important anti-aggregant effect of Buflomedil(R) administrated in these conditions, both on fresh and preserved blood (p < 10(-3)). These results illustrate the potential interest of a morphometric approachto erythrocyte aggregation. They show new pharmacological properties of Buflomedil(R) administered in vitro, which have to be confirmed with a therapeutic administration of the compound.

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Documento generato il 27/10/20 alle ore 11:13:25