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Titolo:
DYNAMICS OF THERMAL MOTIONS WITHIN A LARGE CATALYTIC RNA INVESTIGATEDBY CROSS-LINKING WITH THIOL-DISULFIDE INTERCHANGE
Autore:
COHEN SB; CECH TR;
Indirizzi:
UNIV COLORADO,HOWARD HUGHES MED INST,DEPT CHEM & BIOCHEM BOULDER CO 80309 UNIV COLORADO,HOWARD HUGHES MED INST,DEPT CHEM & BIOCHEM BOULDER CO 80309
Titolo Testata:
Journal of the American Chemical Society
fascicolo: 27, volume: 119, anno: 1997,
pagine: 6259 - 6268
SICI:
0002-7863(1997)119:27<6259:DOTMWA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TETRAHYMENA RIBOZYME REACTION; GUANOSINE-BINDING-SITE; GROUP-I RIBOZYME; ACTIVE-SITE; TERTIARY INTERACTIONS; HAMMERHEAD RIBOZYME; 2'-HYDROXYL GROUPS; GUIDE SEQUENCE; RIBOSOMAL-RNA; 3' END;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Cohen e T.R. Cech, "DYNAMICS OF THERMAL MOTIONS WITHIN A LARGE CATALYTIC RNA INVESTIGATEDBY CROSS-LINKING WITH THIOL-DISULFIDE INTERCHANGE", Journal of the American Chemical Society, 119(27), 1997, pp. 6259-6268

Abstract

Conformational dynamics have been studied in proteins, but the extentof thermal motion within large compact RNA molecules, including ribozymes, has not been systematically explored. Methods are developed for the incorporation of the alkyl-phenyl disulfide 1 and the alkyl thiol 2 at specific ribose 2'-positions within RNA. These groups react undermild conditions to form disulfide cross-links by thiol-disulfide interchange. When incorporated on opposite faces of a short, continuous RNA helix, these reactants do not form a disulfide cross-link. In contrast, in an active 310-nucleotide group I ribozyme prepared by semisynthetic methods, sites separated by a distance of up to 50 Angstrom in the structure are cross-linked at rates only 3- to 15-times slower than cross-linking between proximal sites. Furthermore, ribozymes with normally distant sites held together by a disulfide cross-link retain substantial catalytic activity. These results reveal unexpectedly large thermal motions between domains of a compact folded ribozyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 11:33:56