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Titolo:
HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression
Autore:
Paul, P; Rouas-Freiss, N; Moreau, P; Cabestre, FA; Menier, C; Khalil-Daher, I; Pangault, C; Onno, M; Fauchet, R; Martinez-Laso, J; Morales, P; Villena, AA; Giacomini, P; Natali, PG; Frumento, G; Ferrara, GB; McMaster, M; Fisher, S; Schust, D; Ferrone, S; Dausset, J; Geraghty, D; Carosella, ED;
Indirizzi:
Hop St Louis, Inst Univ Hematol, CEA, DRM,DSV,Ctr Hayem,Serv Rech Hematoimmunol, F-75010 Paris 10, France Hop St Louis Paris France 10 ech Hematoimmunol, F-75010 Paris 10, France Univ Rennes 1, Fac Med, UPRES EA 2233,CR14Y006B, Lab Univ Hematol & Biol Cellules Sanguines, F-35043 Rennes, France Univ Rennes 1 Rennes France F-35043 es Sanguines, F-35043 Rennes, France Hosp 12 Octubre, E-28041 Madrid, Spain Hosp 12 Octubre Madrid Spain E-28041 p 12 Octubre, E-28041 Madrid, Spain Regina Elena Inst CRS, Immunol Lab, Rome, Italy Regina Elena Inst CRS Rome Italy ena Inst CRS, Immunol Lab, Rome, Italy Ctr Biotechnol Avanzate, Immunogenet Lab, I-10132 Genoa, Italy Ctr Biotechnol Avanzate Genoa Italy I-10132 et Lab, I-10132 Genoa, Italy Univ Calif San Francisco, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pathol, Boston, MA 02115 USA New York State Dept Hlth, Roswell Pk Canc Inst, Dept Immunol, Buffalo, NY 14263 USA New York State Dept Hlth Buffalo NY USA 14263 unol, Buffalo, NY 14263 USA Fdn Jean Dausset, F-75010 Paris, France Fdn Jean Dausset Paris France F-75010 ean Dausset, F-75010 Paris, France Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98104 , Seattle, WA 98104 USA
Titolo Testata:
HUMAN IMMUNOLOGY
fascicolo: 11, volume: 61, anno: 2000,
pagine: 1177 - 1195
SICI:
0198-8859(200011)61:11<1177:H--PTA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; NATURAL-KILLER-CELLS; G MESSENGER-RNA; MYELOMONOCYTIC CELLS; INHIBITORY RECEPTOR; HUMAN TROPHOBLASTS; MONOCLONAL-ANTIBODY; NONCLASSICAL MHC; G MOLECULE; G ANTIGEN;
Keywords:
non-classical HLA class I genes; HLA-G, F, -E transcription; HLA-G monoclonal antibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Paul, P Hop St Louis, Inst Univ Hematol, CEA, DRM,DSV,Ctr Hayem,Serv Rech Hematoimmunol, 1 Ave Claude Vellefaux, F-75010 Paris 10, France Hop St Louis 1 Ave Claude Vellefaux Paris France 10 is 10, France
Citazione:
P. Paul et al., "HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression", HUMAN IMMUN, 61(11), 2000, pp. 1177-1195

Abstract

Non-classical MHC class I HLA-E, -F, and -G molecules differ from classical class I histocompatibility antigens by specific patterns of transcription, protein expression, and immunological functions. Restriction of the expression pattern of these non-classical antigens may play a key role in modulation of immune responses during pregnancy and diseases but remains to be additionally defined. A specific component of the second International Conference on HLA-G and the 13th HLA-G Histocompatibility Workshop will be dedicated to the analysis of transcription and expression of non-classical class I genes in normal and pathological tissues. In a first step, referred to asthe preworkshop, we here report the analysis and conclusions of a working group which was constituted to gather and validate optimal reagents and protocols allowing RT-PCR analysis of HLA-E, -F, -G transcript levels and flowcytometry and immunochemistry analysis of HLA-G expression in cells and tissues. As a result of this work, use of specific primers and probes detecting alternative transcripts of HLA-E, -F, and G have been validated in transfected cells expressing differential pattern of HLA class I antigens, Analysis of the specificity and affinity of collected antibodies has allowed definition of reagents to be proposed for immunochemistry and flow cytometry analysis of HLA-G expression in normal and pathological tissues during the workshop. This work has allowed constitution of an extended workshop group which is now initiating analysis of non-classical class I transcription and expression in various cells and tissues, a collective contribution that will additionally refine our view of the expression of these antigens in normal and pathological situations. (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:38:13