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Titolo:
IPM-FISH, a new M-FISH approach using IRS-PCR painting probes: Applicationto the analysis of seven human prostate cell lines
Autore:
Aurich-Costa, J; Vannier, A; Gregoire, E; Nowak, F; Cherif, D;
Indirizzi:
GENSET SA, Dept Cytogenet, CRG, F-91030 Evry, France GENSET SA Evry France F-91030 Dept Cytogenet, CRG, F-91030 Evry, France
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 2, volume: 30, anno: 2001,
pagine: 143 - 160
SICI:
1045-2257(200102)30:2<143:IANMAU>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; IN-SITU HYBRIDIZATION; MOLECULAR CYTOGENETIC ANALYSIS; PAPILLOMAVIRUS TYPE-18 DNA; EPITHELIAL-CELLS; ALLELIC LOSS; GENETIC ALTERATIONS; HUMAN-CHROMOSOMES; INTRAEPITHELIAL NEOPLASIA; HIGH-RESOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Aurich-Costa, J GENSET SA, Dept Cytogenet, CRG, RN7, F-91030 Evry, France GENSET SA RN7 Evry France F-91030 7, F-91030 Evry, France
Citazione:
J. Aurich-Costa et al., "IPM-FISH, a new M-FISH approach using IRS-PCR painting probes: Applicationto the analysis of seven human prostate cell lines", GENE CHROM, 30(2), 2001, pp. 143-160

Abstract

We have developed an alternative multicolor karyotyping technique based onmultiplex fluorescence in situ hybridization (M-FISH) and our own optical device with a specific filter set. The most innovative part of our development is the use of interspersed polymerase chain reaction (IRS-PCR) paintingprobes that show an R-band pattern simultaneous to the combinatorial labeling. This allows us not only to recognize the origin of chromosomal fragments, but to identify the breakpoints as well. We have used this technique toanalyze seven cell lines: four prostate cancer cell lines (CA-HPV-10, LNCaP, DU145, and PC3), and three normal transformed epithelial prostate cell lines (PNT1B, PNT2, and PZ-HPV-7). In order to validate our IRS-PCR multiplex FISH (IPM-FISH) technique and to complement the results, we applied comparative genomic hybridization (CGH) and FISH analysis, showing good correlation with the IPM-FISH results. To date, molecular and cytogenetic studies have identified several chromosomal regions that are altered in human prostate cancer; several candidate genes have been suggested. However, reliable markers for predicting the aggressiveness of early prostate cancer are not yet available. Our results show several common, unbalanced rearrangements inthe cell lines. These rearrangements are similar to regions already implicated in prostate cancer, validating these cell lines as a good model system. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 14:56:35