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Titolo:
Keratin 23 (K23), a novel acidic keratin, is highly induced by histone deacetylase inhibitors during differentiation of pancreatic cancer cells
Autore:
Zhang, JS; Wang, L; Huang, H; Nelson, M; Smith, DI;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Div Expt Pathol, Ctr Canc, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Canc, Rochester, MN 55905 USA
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 2, volume: 30, anno: 2001,
pagine: 123 - 135
SICI:
1045-2257(200102)30:2<123:K2(ANA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-BUTYRATE; TRANSCRIPTIONAL REPRESSION; GROWTH-INHIBITION; LINES; APOPTOSIS; CARCINOMA; MECP2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, DI Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Div Expt Pathol, Ctr Canc, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1stSt SW Rochester MN USA 55905 05 USA
Citazione:
J.S. Zhang et al., "Keratin 23 (K23), a novel acidic keratin, is highly induced by histone deacetylase inhibitors during differentiation of pancreatic cancer cells", GENE CHROM, 30(2), 2001, pp. 123-135

Abstract

Sodium butyrate (NaBu) was shown to induce differentiation and apoptosis in the human pancreatic cancer cell line AsPC-I. A suppression subtractive hybridization-based technique was used to identify genes induced by NaBu. A novel cDNA was found to be highly up-regulated in AsPC-I cells in response to NaBu. The gene expresses a 1.65-kb mRNA encoding a protein with an open reading frame of 422 amino acids. It has an intermediate filament signaturesequence and extensive homology to type I keratins. Sequence comparison with known keratins indicated that the gene shares 42-46% amino acid identityand 60-65% similarity within the ct-helical rod domain. The gene is named K23 (for human type I Keratin 23, KRT23). K23 mRNA was highly induced by NaBu in different pancreatic cancer cells. Trichostatin A (TSA), a potent andspecific inhibitor of histone deacetylase, similarly induced K23 mRNA expression. Treatment with either actinomycin D or cycloheximide efficiently blocked the induction of K23 mRNA by NaBu/TSA. These results indicate that K23 mRNA induction by NaBu or TSA is a downstream event of histone hyperacetylation. We also demonstrated that expression of p21(WAF1/CIP1) antisense RNA efficiently blocked the induction of K23 mRNA induced by NaBu. Our results suggest that K23 is a novel member of the acidic keratin family that is induced in pancreatic cancer cells undergoing differentiation by a mechanisminvolving histone hyperacetylation. p21(WAF1/CIP1) serves as an important mediator during the induction process of K23 by NaBu. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:25:01