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Titolo:
Identification of novel deletion regions on chromosome arms 2q and 6p in breast carcinomas by amplotype analysis
Autore:
Piao, Z; Lee, KS; Kim, H; Perucho, M; Malkhosyan, S;
Indirizzi:
Burnham Inst, Oncogene & Tumor Suppressor Gene Program, La Jolla, CA 92037USA Burnham Inst La Jolla CA USA 92037 or Gene Program, La Jolla, CA 92037USA Yonsei Univ, Coll Med, Dept Surg, Seoul, South Korea Yonsei Univ Seoul South Korea , Coll Med, Dept Surg, Seoul, South Korea Yonsei Univ, Coll Med, Dept Pathol, Seoul, South Korea Yonsei Univ SeoulSouth Korea Coll Med, Dept Pathol, Seoul, South Korea
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 2, volume: 30, anno: 2001,
pagine: 113 - 122
SICI:
1045-2257(200102)30:2<113:IONDRO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; MOLECULAR CYTOGENETIC ANALYSIS; POLYMERASE-CHAIN-REACTION; ALLELIC LOSSES; COLORECTAL-CANCER; LUNG-CARCINOMA; CELL CARCINOMA; BRCA2; PCR; HETEROZYGOSITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Malkhosyan, S Burnham Inst, Oncogene & Tumor Suppressor Gene Program, 10901 N Torrey Pines Rd, La Jolla, CA 92037 USA Burnham Inst 10901 N Torrey Pines Rd La Jolla CA USA 92037 A
Citazione:
Z. Piao et al., "Identification of novel deletion regions on chromosome arms 2q and 6p in breast carcinomas by amplotype analysis", GENE CHROM, 30(2), 2001, pp. 113-122

Abstract

DNA fingerprinting by arbitrarily primed PCR (AP-PCR) was employed to identify molecular genetic alterations in 37 primary breast carcinomas. AP-PCR is a PCR-based technique that uses only one primer of arbitrary sequence that generates a molecular karyotype (amplotype) of tumors. The breast canceramplotype generated with two arbitrary primers (MCGI and Blue) showed a relatively high frequency (more than 20% of the tumors) of gains at chromosomes 1, 4, and 8, and of losses at chromosomes 2, 4, 6, 9, 10, 11, 13, and the X chromosome. We further analyzed the regions most commonly gained at chromosome 8 (47%) and lost at chromosomes 2 (38%) and 6 (49%) by determining the subchromosomal localization of the fingerprint bands from these chromosomes. The region of gain at chromosome 8 was mapped at 8q24.1, close to MYCBand MCGI-AI was assigned to chromosome band 2q22, and band Blue-J was assigned to 6p21. Common losses of these chromosomal regions have not been described for breast cancer. To map these deletion regions more precisely, we performed loss of hererozygosity (LOH) analysis by microallelotyping on 20 of the 37 cancers previously analyzed by AP-PCR and another additional 52 breast carcinomas. The results suggest that the regions at 2q21-24 and 6p21-23 may harbor novel tumor suppressor genes for breast cancer. LOH at 2q21-24 (D2S2304) was more frequent in high-grade tumors (59%) than in low-grade rumors (29) (P = 0.03). This suggests that this genetic alteration may be associated with tumor progression and shows the power of the amplotype approach in detecting novel genetic alterations that are useful as clinical parameters of breast cancer. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:06:54