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Titolo:
Biochemical and immunohistological changes in the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mouse
Autore:
Araki, T; Mikami, T; Tanji, H; Matsubara, M; Imai, Y; Mizugaki, M; Itoyama, Y;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Hosp, Dept Pharmaceut Sci, Sendai, Miyagi, Japan Tohoku Univ Hosp Sendai Miyagi Japan armaceut Sci, Sendai, Miyagi, Japan Tohoku Univ, Sch Med, Dept Neurol, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 ept Neurol, Sendai, Miyagi 980, Japan Tohoku Univ, Sch Med, Dept Internal Med 2, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 rnal Med 2, Sendai, Miyagi 980, Japan
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
fascicolo: 3, volume: 12, anno: 2001,
pagine: 231 - 238
SICI:
0928-0987(200101)12:3<231:BAICIT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOPAMINE UPTAKE SITES; PROGRESSIVE SUPRANUCLEAR PALSY; D2 RECEPTOR STATUS; LONG-TERM CHANGES; RAT-BRAIN; PARKINSONS-DISEASE; AUTORADIOGRAPHIC VISUALIZATION; SELECTIVE DESTRUCTION; SUBSTANTIA-NIGRA; D-1 RECEPTORS;
Keywords:
MPTP; dopaminergic receptors; dopamine uptake sites; receptor autoradiography; immunohistochemistry; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Araki, T Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol &Therapeut, Aoba Ku, Aoba Yama, Sendai, Miyagi 9808578, Japan Tohoku Univ Aoba Yama Sendai Miyagi Japan 9808578 9808578, Japan
Citazione:
T. Araki et al., "Biochemical and immunohistological changes in the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mouse", EUR J PH SC, 12(3), 2001, pp. 231-238

Abstract

We investigated neurochemically and neuropathologically the utility of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice as a model ofParkinson's disease. The changes in dopamine D-1 and D-2 receptors and dopamine uptake sites were determined by quantitative autoradiography using [H-3]SCW23390, [H-3]raclopride and [H-3]mazindol, respectively. Dopamine and 3,4-dihydroxyphenyl acetic acid (DOPAC) contents in the striatum were measured by high-performance liquid chromatography. The distribution of nigral neurons and reactive astrocytes was determined by immunohistochemical staining with antibody against tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP). The mice received four intraperitoneal injections of MPTP(10 mg/kg) at 1-h intervals and then the brains were analyzed at 3 and 7 days after the treatments. No significant change in dopamine D-1 receptors was observed in the striatum and substantia nigra after acute treatment with MPTP. Dopamine D-2 receptors were reduced significantly in the substantianigra only 7 days after the MPTP treatment, whereas striatum showed no significant change in the binding throughout the experiments. In contrast, dopamine uptake sites were reduced markedly in the striatum and substantia nigra 3 and 7 days after the MPTP treatment. Dopamine and DOPAC content were also reduced in the striatum 3 and 7 days after the MPTP treatment. An immunohistochemical study indicated a loss of the number of TH-positive neurons in the substantia nigra 7 days after the MPTP treatment. In contrast, numerous GFAP-positive astrocytes were evident in the striatum 7 days after the MPTP treatment. These results provide valuable information for the pathogenesis of acute stage of Parkinson's disease. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:23:44