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Titolo:
Characterization of cytokine interactions by flow cytometry and factorial analysis
Autore:
Case, J; Rice, A; Wood, J; Gaudry, L; Vowels, M; Nordon, RE;
Indirizzi:
Univ New S Wales, Grad Sch Biomed Sci, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 i, Sydney, NSW 2052, Australia Sydney Childrens Hosp, Childrens Canc Inst Australia Med Res, Sydney, NSW,Australia Sydney Childrens Hosp Sydney NSW Australia ed Res, Sydney, NSW,Australia Prince Wales Hosp, Dept Haematol, Sydney, NSW, Australia Prince Wales Hosp Sydney NSW Australia Haematol, Sydney, NSW, Australia Sydney Childrens Hosp, Dept Paediat Haematol Oncol, Sydney, NSW, AustraliaSydney Childrens Hosp Sydney NSW Australia Oncol, Sydney, NSW, Australia
Titolo Testata:
CYTOMETRY
fascicolo: 1, volume: 43, anno: 2001,
pagine: 69 - 81
SICI:
0196-4763(20010101)43:1<69:COCIBF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; HIGH-DOSE CHEMOTHERAPY; HUMAN CORD-BLOOD; PROGENITOR CELLS; IN-VITRO; C-KIT; THROMBOPOIETIN; STEM; ERYTHROPOIETIN; INTERLEUKIN-3;
Keywords:
hematopoietic stem cells; fetal blood; factorial experimental design; flow cytometry; hematopoietic growth factors; cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Nordon, RE Univ New S Wales, Grad Sch Biomed Sci, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 NSW 2052, Australia
Citazione:
J. Case et al., "Characterization of cytokine interactions by flow cytometry and factorial analysis", CYTOMETRY, 43(1), 2001, pp. 69-81

Abstract

Background: Multiple cytokines are required for the growth and developmentof hematopoietic cells. The effect of many cytokines depends on the activity of other signaling pathways. These interactions are quantified using factorial experimental design and analysis. Methods: Human umbilical cord blood (HUCB) CD34(+) cells were cultured in fully defined media containing various combinations of recombinant cytokines as defined by resolution IV factorial (2(IV)(7-3)) Or full factorial (2(4)) design experiments. The cytokines studied were stem cell factor (SCF), interleukin (IL)-3, megakaryocyte growth and development factor (MGDF), granulocyte-colony stimulating factor (G-CSF), Fit-3 ligand, IL-6, IL-11, and erythropoietin (EPO). In vitro cell divisions were tracked by staining CD34() cells with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester, followed by now cytometric analysis at 4 days of culture. In separate experiments, lineage commitment and differentiation were determined at 7 days by immunophenotype. Results: In addition to the main effects of single cytokines, cytokine interactions were identified. There was a negative interaction between IL-3 and MGDF that resulted in a less than additive effect of these factors on erythroid and megakaryocytic development. The effect of Fit-3 ligand and SCF factor on CD34(+) cell production was also less than additive, although the response to both cytokines was greater than single cytokines. The only positive interaction that was identified was between EPO and SCF, which resulted in the synergistic production of erythroid cells. Conclusions: Factorial analysis provides a powerful methodology to study the integration of multiple signals at the cellular and molecular level. Cyometry 43:69-81, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:23:07