Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NASA/American Cancer Society high-resolution flow cytometry project - III.Multiparametric analysis of DNA content and electronic nuclear volume in human solid tumors
Autore:
Krishan, A; Wen, JH; Thomas, RA; Sridhar, KS; Smith, WI;
Indirizzi:
Univ Miami, Sch Med, Dept Radiat Oncol, Div Expt Therapeut, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 ol, Div Expt Therapeut, Miami, FL 33101 USA RATCOM Inc, Miami, FL USA RATCOM Inc Miami FL USARATCOM Inc, Miami, FL USA Univ Miami, Sch Med, Dept Med, Div Hematol Oncol, Miami, FL USA Univ Miami Miami FL USA Med, Dept Med, Div Hematol Oncol, Miami, FL USA Suburban Hosp, Dept Pathol, Bethesda, MD USA Suburban Hosp Bethesda MD USA burban Hosp, Dept Pathol, Bethesda, MD USA
Titolo Testata:
CYTOMETRY
fascicolo: 1, volume: 43, anno: 2001,
pagine: 16 - 22
SICI:
0196-4763(20010101)43:1<16:NCSHFC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE; DIFFERENTIATION;
Keywords:
aneuploidy; DNA content; DNA index; electronic nuclear volume; flow cytometry; hypodiploid; tumors; nuclear packing efficiency; metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Krishan, A Univ Miami, Sch Med, Dept Radiat Oncol, Div Expt Therapeut, POB016960, Miami, FL 33101 USA Univ Miami POB 016960 Miami FL USA 33101 0, Miami, FL 33101 USA
Citazione:
A. Krishan et al., "NASA/American Cancer Society high-resolution flow cytometry project - III.Multiparametric analysis of DNA content and electronic nuclear volume in human solid tumors", CYTOMETRY, 43(1), 2001, pp. 16-22

Abstract

Background: The NASA/American Cancer Society (ACS) flow cytometer can simultaneously measure electronic nuclear volume (ENV) and DNA content of nuclei. The preceding articles in this volume ("NASA/American Can cer Society High-Resolution Flow Cytometer Project-I") described the schematics, performance, and procedures used for the preparation of nuclei for analysis on thisunit. In the present article, we describe the analysis of selected human tumors using the ratio of ENV/DNA content (nuclear packing efficiency [NPE]). Methods: Tumor specimens (frozen) were minced with scalpels and stained with 1-10 mug/ml of 4',6-diamidino-2-phenylindole (DAPI) dihydrochloride at pH 6.0 -7.2. Trout erythrocytes were used as internal standards. Data on ENVand DNA content were collected in list mode files. Propidium iodide-stained nuclei, analyzed on a Coulter XL cytometer, were used for comparison. Results: Simultaneous measurement of ENV and DNA makes it possible to discriminate between hypodiploid or hyperdiploid tumor cells, as well as to differentiate between near-diploid aneuploid and diploid cells on the basis oftheir increased ENV. The NPE ratio is a valuable parameter for the detection of small quantities of tumor cells, separating overlapping diploid and aneuploid populations for cell cycle analysis and characterizing the level of differentiation in some tumors. Conclusion: NPE analysis provides unique measuring capabilities for the study of human solid tumors by flow cytometry. Cytometry 43:16-22, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:20:13