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Titolo:
Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification
Autore:
Shibli, RA; Al-Juboory, KH;
Indirizzi:
Jordan Univ Sci & Technol, Ctr Agr Biotechnol, Irbid, Jordan Jordan Univ Sci & Technol Irbid Jordan tr Agr Biotechnol, Irbid, Jordan Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 es & Environm Sci, Urbana, IL 61801 USA
Titolo Testata:
CRYO-LETTERS
fascicolo: 6, volume: 21, anno: 2000,
pagine: 357 - 366
SICI:
0143-2044(200011/12)21:6<357:CO'O(E>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWN APICAL MERISTEMS; WASABI WASABIA-JAPONICA; PLANT-REGENERATION; LIQUID-NITROGEN; VITRO; EMBRYOGENESIS; DESICCATION; INVITRO; TOLERANCE; GERMPLASM;
Keywords:
cryopreservation; olive; Olea europea L.; encapsulation-dehydration; encapsulation-vitrification; somatic embryos;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Shibli, RA Jordan Univ Sci & Technol, Ctr Agr Biotechnol, Irbid, Jordan Jordan Univ Sci & Technol Irbid Jordan echnol, Irbid, Jordan
Citazione:
R.A. Shibli e K.H. Al-Juboory, "Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification", CRYO-LETT, 21(6), 2000, pp. 357-366

Abstract

Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification. In the encapsulation-dehydration procedure, a maximum of 48% embryo survival was obtained when bead moisture content was decreased to 21.1% after 4 h dehydration. Preculture of embryos for 4 d in medium containing 0.75 to 1.25 M sucrose produced higher (40 to 34%, respectively) regrowth after cryopreservation using encapsulation-dehydration procedure. Dehydration of beads for 3 h in PVS2 ensured higher survival (64%) of encapsulated-vitrified and cryopreserved (EVN) somatic embryos. Thermal treatment of embryogenic callus for 1 d at 30 degreesC was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos. Plantlets produced from control and cryopreserved embryos were phenotypically similar.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:34:51