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Titolo:
The AIDA autotransporter system is associated with F18 and Stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea
Autore:
Niewerth, U; Frey, A; Voss, T; Le Bouguenec, C; Baljer, G; Franke, S; Schmidt, MA;
Indirizzi:
Univ Munster, Zentrum Mol Biol Entzundung, Inst Infektiol, Munster, Germany Univ Munster Munster Germany tzundung, Inst Infektiol, Munster, Germany Univ Giessen, Inst Hyg & Infekt Krankheiten Tiere, D-6300 Giessen, GermanyUniv Giessen Giessen Germany D-6300 eiten Tiere, D-6300 Giessen, Germany Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, Paris, France Inst Pasteur Paris France thogenie Bacterienne Muqueuses, Paris, France
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 1, volume: 8, anno: 2001,
pagine: 143 - 149
SICI:
1071-412X(200101)8:1<143:TAASIA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFUSE ADHERENCE; MOLECULAR CHARACTERIZATION; STRAIN-2787 O126-H27; FIMBRIAL ANTIGENS; ADHESIN; ENTEROTOXINS; GENES; EXPRESSION; PREVALENCE; PRECURSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt, MA ZMBE, Inst Infektiol, Von Esmarch Str 56, D-48149 Munster, Germany ZMBE Von Esmarch Str 56 Munster Germany D-48149 ster, Germany
Citazione:
U. Niewerth et al., "The AIDA autotransporter system is associated with F18 and Stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea", CL DIAG LAB, 8(1), 2001, pp. 143-149

Abstract

Pathogenic Escherichia call strains are known to cause edema disease (ED) and postweaning diarrhea (PWD) in piglets. Although the exact mechanisms ofpathogenicity that lead to ED-PWD remain to be elucidated, E,coli-borne Shiga-like toxin and adhesion-mediating virulence factors such as F18 adhesinor F4 fimbriae are believed to play a central role in ED-PWD, In light of these observations we investigated whether another E, call adhesin, the plasmid-encoded AII)A (adhesin involved in diffuse adherence) might also be present in ED-PWD-causing E, coli isolates, For rapid screening for the AIDA system in large numbers of isolates, a multiplex PCR method along with a duplex Western blot procedure was developed. When screening 104 strains obtained from pigs with or without ED-PWD, we observed a high prevalence of the AIDA operon in porcine E, coli isolates, with over 25% of all strains beingAIDA positive, and we could demonstrate a significant association of the intact AIDA gene (orfB) with ED-PWD, while defects in orfB were associated with the absence of disease. Although our data hint toward a contribution ofAIDA to ED-PWD, further studies will be necessary since the presence of the AIDA genes was also associated with the presence of the Shiga-like toxin and F18 adhesin genes, two reported virulence factors for ED-PWD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 02:32:33