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Titolo:
Putative role of basement membrane for dentinogenesis in the mesenchyme ofmurine dental papillae in vitro
Autore:
Kikuchi, H; Amano, H; Yamada, S;
Indirizzi:
Showa Univ, Sch Dent, Dept Pharmacol, Shinagawa Ku, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 macol, Shinagawa Ku, Tokyo 1428555, Japan
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 1, volume: 303, anno: 2001,
pagine: 93 - 107
SICI:
0302-766X(200101)303:1<93:PROBMF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; DIFFERENTIATION IN-VITRO; LATENT TGF-BETA; LAMININ-A-CHAIN; EXTRACELLULAR-MATRIX; ODONTOBLAST DIFFERENTIATION; TOOTH DEVELOPMENT; BINDING-PROTEIN; EPITHELIAL MORPHOGENESIS; EMBRYONIC-DEVELOPMENT;
Keywords:
isolated incisor dental papilla; artificial substratum; basement membrane components; transforming growth factor-beta(1); Odontoblast differentiation; rat (Wistar);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Kikuchi, H Showa Univ, Sch Dent, Dept Pharmacol, Shinagawa Ku, Hatanodai 1-5-8, Tokyo1428555, Japan Showa Univ Hatanodai 1-5-8 Tokyo Japan 1428555 o1428555, Japan
Citazione:
H. Kikuchi et al., "Putative role of basement membrane for dentinogenesis in the mesenchyme ofmurine dental papillae in vitro", CELL TIS RE, 303(1), 2001, pp. 93-107

Abstract

In a new culture-conditioning system of agar-coated mesenchyme of isolatedincisor dental papillae, dentinogenesis has been induced adjacent to an agar substratum that functions as a foothold for cell immobilisation. To elucidate the role of the basement membrane (BM) in dentinogenesis, we have examined the way in which dentinogenesis depends upon BM components or transforming growth factor (TGF)-beta (1) in this system. At the mesenchymal-epithelial junction of odontogenic organs (cut incisor tooth germs), TGF-beta (1) visibly increased in the BM during incubation. In isolated dental papillae, BM components were synthesised and deposited at aligned peripheral cellsof the explants, together with an increasing amount of TGF-beta (1). Thesecomponents were not assembled into extracellular matrix (ECM)-absorbed agar adjacent to explants, although dentinogenesis proceeded in the presence of pericellular BM components associated with TGF-beta (1). When signalling via TGF-beta type II receptors was blocked, neither ECM production nor dentinogenesis was observed but explants partially detached from the agar surface, presumably as a result of the suppressed production of ECM, since attachment was retained by pre-coating explants with artificial matrices. Rescueexperiments showed that TGF-beta (1) regulated dentinogenesis through ECM production. With regard to BM components, inducible dentinogenesis was Arg-Cly-Asp (RGD)-dependent. Thus, pericellular BM components associated with TGF-beta (1) and an ECM-absorbed agar substratum, which affects dentinogenesis, synergistically play a role similar to that of BM components in vivo. The BM therefore serves as a structural meshwork that acts as a foothold forcell immobilisation; its components act as ligands for RGD-dependent cell adhesion and it stores TGF-beta (1), which regulates ECM production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:27:22