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Titolo:
Rat seminiferous epithelium contains a unique junction (ectoplasmic specialization) with signaling properties both of cell/cell and cell/matrix junctions
Autore:
Mulholland, DJ; Dedhar, S; Vogl, AW;
Indirizzi:
Univ British Columbia, Dept Anat, Vancouver, BC V6H 3Z6, Canada Univ British Columbia Vancouver BC Canada V6H 3Z6 ver, BC V6H 3Z6, Canada Univ British Columbia, Dept Biochem, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 1, volume: 64, anno: 2001,
pagine: 396 - 407
SICI:
0006-3363(200101)64:1<396:RSECAU>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL ADHESION KINASE; MAMMALIAN SERTOLI CELLS; SPERMATOGENIC CELLS; ACTIN; ALPHA-6-BETA-1; CADHERINS; VINCULIN; RECEPTOR; PROTEINS; LAMININ;
Keywords:
developmental biology; gametogenesis; Sertoli cells; signal transduction; spermatid; spermatogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Mulholland, DJ Jack Bell Res Ctr, 2660 Oak St, Vancouver, BC V6H 3Z6, Canada Jack Bell Res Ctr 2660 Oak St Vancouver BC Canada V6H 3Z6 a
Citazione:
D.J. Mulholland et al., "Rat seminiferous epithelium contains a unique junction (ectoplasmic specialization) with signaling properties both of cell/cell and cell/matrix junctions", BIOL REPROD, 64(1), 2001, pp. 396-407

Abstract

The seminiferous epithelium contains unique actin related cell-cell junctions, termed ectoplasmic specializations (ESs). Turnover of these junctions is fundamental to sperm release and to movement of spermatocytes from basalto adluminal compartments of the epithelium during spermatogenesis. In this study we report several novel observations related to the spatial and temporal distribution of integrin-related signaling molecules at ESs. We confirm the presence of beta (1)-integrin at these sites and further demonstrateco-localization of integrin linked kinase (ILK). beta (1)-Integrin and ILKwere shown by immunoprecipitation to associate in whole cell lysates of seminiferous epithelium. This observation provides the first evidence for a direct beta (1)-integrin/ILK interaction in noncultured epithelium. Pan-cadherin and beta -catenin antibodies did not react at ESs. Rather, antibodies reacted with desmosome-like junctions that are present both at basal junctional complexes between Sertoli cells and at sites of attachment to spermatogenic cells. Focal adhesion kinase (FAK), a known integrin-associated molecule, did not codistribute with beta (1)-integrins and did not associate with these adhesion molecules in immunoprecipitation studies. Although FAK wasexpressed in the epithelium, it appeared to be limited to the cytoplasm ofearly spermatogenic cells. Significantly, polyclonal antibodies against phosphotyrosine-containing residues reacted strongly at ESs, with highest levels detected during sperm release and turnover of basal junction complexes. Our observations indicate that ESs share cell signaling features both of cell-cell junctions and of cell-extracellular matrix junctions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 23:44:56