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Titolo:
BINDING OF HUMAN PERIPHERAL-BLOOD POLYMORPHONUCLEAR LEUKOCYTES TO E-SELECTIN (CD62E) DOES NOT PROMOTE THEIR ACTIVATION
Autore:
REPO H; ROCHON YP; SCHWARTZ BR; SHARAR SR; WINN RK; HARLAN JM;
Indirizzi:
UNIV HELSINKI,DEPT BACTERIOL & IMMUNOL,HAARTMAN INST,POB 21,HAARTMANINKATU 3 HELSINKI 00014 FINLAND UNIV WASHINGTON,DEPT MED SEATTLE WA 98195 UNIV WASHINGTON,DEPT ANESTHESIOL SEATTLE WA 98195 UNIV WASHINGTON,DEPT SURG SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHYSIOL BIOPHYS SEATTLE WA 98195 UNIV HELSINKI,CENT HOSP,DEPT MED HELSINKI FINLAND UNIV HELSINKI,CENT HOSP,DIV INFECT DIS HELSINKI FINLAND
Titolo Testata:
The Journal of immunology
fascicolo: 2, volume: 159, anno: 1997,
pagine: 943 - 951
SICI:
0022-1767(1997)159:2<943:BOHPPL>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW CYTOMETRIC DETERMINATION; HUMAN ENDOTHELIAL-CELLS; REGULATION IN-VIVO; ADHESION MOLECULE-1; P-SELECTIN; NEUTROPHIL ADHESION; UP-REGULATION; CHEMOTACTIC FACTORS; CD11B CD18; MAC-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
H. Repo et al., "BINDING OF HUMAN PERIPHERAL-BLOOD POLYMORPHONUCLEAR LEUKOCYTES TO E-SELECTIN (CD62E) DOES NOT PROMOTE THEIR ACTIVATION", The Journal of immunology, 159(2), 1997, pp. 943-951

Abstract

E-selectin (CD62E) is a cytokine-inducible endothelial cell adhesion molecule that tethers polymorphonuclear leukocytes (PMNs) and supportsPMN rolling under conditions of flow. We examined whether interactionof PMNs with E-selectin also leads to activation of CD11b/CD18 (Mac-1, alpha(M) beta(2)), an event that can promote firm adhesion. PMNs were added to monolayers of IL-1 beta-activated HUVECs and Chinese hamster ovary (CHO) cells transfected with E-selectin cDNA. PMN activation was assessed by 1) increased CD11b/CD18 surface expression, 2) appearance of activation epitope on CD11b/CD18 (CD11b) detected by mAb CBRM1/5, and 3) decreased L-selectin (CD62L) expression, as determined by flow cytometry. Both adherent and nonadherent supernatant PMNs became activated on IL-1 beta-pretreated HUVECs. This activation was not affected by CD62E-blocking mAb P6E2. The activation state of PMNs adhered toCHO cells transfected with E-selectin cDNA was not increased over background and was similar to that of PMNs exposed to parent CHO cells. The findings were confirmed using confocal microscopy, which allowed staining of the cells for CD11b in situ. In concert, the results suggest that PMN binding to E-selectin does not elicit inter-receptor signaling that could result in strengthening of PMN adhesion to endothelium.

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Documento generato il 23/01/21 alle ore 03:16:39