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Titolo:
Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2
Autore:
Zhou, SD; Davis, FC; Ingram, LO;
Indirizzi:
Univ Florida, Dept Microbiol & Cell Sci, Inst Food & Agr Sci, Gainesville,FL 32611 USA Univ Florida Gainesville FL USA 32611 & Agr Sci, Gainesville,FL 32611 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 1, volume: 67, anno: 2001,
pagine: 6 - 14
SICI:
0099-2240(200101)67:1<6:GIAEAE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; ZYMOMONAS-MOBILIS; SACCHAROMYCES-CEREVISIAE; CELLULOSE DEGRADATION; BETA-GLUCOSIDASE; CELLULASES; BACTERIAL; BIOMASS; YEAST; CONVERSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Ingram, LO Univ Florida, Dept Microbiol & Cell Sci, Inst Food & Agr Sci, POB 110700, Gainesville, FL 32611 USA Univ Florida POB 110700 Gainesville FLUSA 32611 , FL 32611 USA
Citazione:
S.D. Zhou et al., "Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2", APPL ENVIR, 67(1), 2001, pp. 6-14

Abstract

The development of methods to reduce costs associated,vith the solubilization of cellulose is essential for the utilization of lignocellulose as a renewable feedstock for fuels and chemicals. One promising approach is the genetic engineering of ethanol-producing microorganisms that also produce cellulase enzymes during fermentation. By starting with an ethanologenic derivative (strain P2) of Klebsiella oxytoca M5A1 with the native ability to metabolize cellobiose, the need for supplemental P-glucosidase was previously eliminated. In the current study, this approach has been extended by addinggenes encoding endoglucanase activities. Genes celY and celZ from Erwinia chrysanthemi have been functionally integrated into the chromosome of P2 using surrogate promoters from Zymomonas mobilis for expression. Both were secreted into the extracellular milieu, producing more than 20,000 endoglucanase units (carboxymethyl cellulase activity) per liter of fermentation broth. During the fermentation of crystalline cellulose with low levels of commercial cellulases of fungal origin, these new strains produced up to 22% more ethanol than unmodified P2. Most of the beneficial contribution was attributed to CelY rather than to CelZ. These results suggest that fungal enzymes with substrate profiles resembling CelY (preference for long-chain polymers and lack of activity on soluble cello oligosaccharides of two to five glucosyl residues) may be limiting in commercial cellulase preparations.

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Documento generato il 01/04/20 alle ore 20:24:19