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Titolo:
The use of long PCR to confirm three common alleles at the CYP2A6 locus and the relationship between genotype and smoking habit
Autore:
Gu, DF; Hinks, LJ; Morton, NE; Day, INM;
Indirizzi:
Southampton Univ Hosp, Sch Med, Res Div Human Genet, Southampton SO16 6YD,Hants, England Southampton Univ Hosp Southampton Hants England SO16 6YD D,Hants, England Chinese Acad Med Sci, Cardiovasc Inst, Fu Wai Hosp, Beijing 100037, Peoples R China Chinese Acad Med Sci Beijing Peoples R China 100037 037, Peoples R China Peking Union Med Coll, Beijing 100037, Peoples R China Peking Union Med Coll Beijing Peoples R China 100037 37, Peoples R China
Titolo Testata:
ANNALS OF HUMAN GENETICS
, volume: 64, anno: 2000,
parte:, 5
pagine: 383 - 390
SICI:
0003-4800(200009)64:<383:TUOLPT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-P450 2A6 CYP2A6; HUMAN LIVER-MICROSOMES; COUMARIN 7-HYDROXYLATION; NICOTINE METABOLISM; GENE; DELETION; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Day, INM Univ Southampton, Sch Med, Res Div Human Genet, Duthie Bldg,MP808,Tremona Rd, Southampton SO16 6YD, Hants, England Univ Southampton Duthie Bldg,MP808,Tremona Rd Southampton Hants England SO16 6YD
Citazione:
D.F. Gu et al., "The use of long PCR to confirm three common alleles at the CYP2A6 locus and the relationship between genotype and smoking habit", ANN HUM GEN, 64, 2000, pp. 383-390

Abstract

Long PCR follow ed by nested PCR has previously been used to determine CYP2A6 160H alleles; but the method proved unreliable. We have optimized this approach in a DNA bank of 1032 subjects rage range 59-74 years) to give reliable results, yielding indirect molecular evidence and very strong statistical evidence of hitherto unrecognized common alleles (designated O) recalcitrant to the long PCR. Coding three alleles (160L, 160H and O) and an approach to association analysis originally developed to deal with null allelesimplicit in ABO blood group phenotyping, the contribution of 160H (functionally null) to reduced smoking habit has been clearly measured for the first time, unconfounded by alleles null to the long PCR. The most significant findings (p < 0.01) are that the possession of a 160H allele, compared withnot possessing a 160H allele, is associated with a mean age of starting regular smoking 3 Sears later (95% CI+/-1.93 years, average start age 20-21 years rather than 17-18 years); and that the average likelihood of quitting smoking at any time is 1.75 fold (95% CI. 1.17-2.61) for those possessing an 160H allele compared with those who hare no 160H allele. This suggests that a smoking subject with a genotype predicted to confer 50% of the abilityto eliminate nicotine via the CYP2A6 pathways has almost twice the likelihood of quitting smoking.

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Documento generato il 05/07/20 alle ore 13:14:03