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Titolo:
Perfluorohexane attenuates proinflammatory and procoagulatory response of activated monocytes and alveolar macrophages
Autore:
Koch, T; Ragaller, M; Haufe, D; Hofer, A; Grosser, M; Albrecht, DM; Kotzsch, M; Luther, T;
Indirizzi:
Univ Hosp Carl Gustav Carus, Dept Anesthesiol & Intens Care Med, D-01307 Dresden, Germany Univ Hosp Carl Gustav Carus Dresden Germany D-01307 307 Dresden, Germany Tech Univ Dresden, Inst Pathol, D-8027 Dresden, Germany Tech Univ DresdenDresden Germany D-8027 Pathol, D-8027 Dresden, Germany Tech Univ Dresden, Inst Anat, D-8027 Dresden, Germany Tech Univ Dresden Dresden Germany D-8027 t Anat, D-8027 Dresden, Germany
Titolo Testata:
ANESTHESIOLOGY
fascicolo: 1, volume: 94, anno: 2001,
pagine: 101 - 109
SICI:
0003-3022(200101)94:1<101:PAPAPR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARTIAL LIQUID VENTILATION; RESPIRATORY-DISTRESS SYNDROME; NEUTROPHIL-MEDIATED INJURY; ACUTE LUNG INJURY; TISSUE FACTOR; BRONCHOALVEOLAR LAVAGE; ENDOTHELIAL-CELLS; INITIAL EXPERIENCE; FACTOR-VII; PERFLUOROCARBON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Koch, T Univ Hosp Carl Gustav Carus, Dept Anesthesiol & Intens Care Med, Fetscherstr 74, D-01307 Dresden, Germany Univ Hosp Carl Gustav Carus Fetscherstr 74 Dresden Germany D-01307
Citazione:
T. Koch et al., "Perfluorohexane attenuates proinflammatory and procoagulatory response of activated monocytes and alveolar macrophages", ANESTHESIOL, 94(1), 2001, pp. 101-109

Abstract

Background: A number of studies have demonstrated the effectiveness of liquid ventilation with perfluorocarbons in improving pulmonary function in acute respiratory distress syndrome. Although it is known that perfluorocarbon-associated gas exchange facilitates lung mechanics and oxygenation, the complete mechanism by which perfluorocarbons exert their beneficial effects in acute lung injury still remains unclear. Possibly, an influence of perfluorocarbons on proinflammatory and procoagulant features of monocytic cellspresent in the alveolar space, such as alveolar macrophages (AMs), may be involved. Therefore, we examined in an in vitro model the effects of perfluorocarbon on both activated mononuclear blood cells (MBCs) and AMs by monitoring the expression of interleukin (IL)-1 beta, tumor necrosis factor (TNF)alpha, and tissue factor (TF). Methods: Mononuclear blood cells, obtained from peripheral blood of healthy volunteers, or AMs from diagnostic bronchoalveolar lavage were stimulatedby incubation with Lipopolysaccharide in the presence of different amountsof perfluorohexane, which was devoid of cytotoxicity. Results: Using both video-enhanced contrast and electron microscopy, the authors observed that perfluorohexane droplets were phagocytosed by activated monocytes as web as by in vitro-cultured AMs within 1-3 h. After lipopolysaccharide stimulation of monocytes or AMs, we observed a down-regulation of TF mRNA and a significant inhibition (P < 0.05) of cellular TF antigen byperfluorohexane. In addition, the concentration of both IL-1<beta> and TNFalpha in the supernatant of lipopolysaccharide-stimulated MBC was significantly decreased (P < 0.01) by perfluorohexane compared with controls without perfluorohexane. By preincubation of lipopolysaccharide-containing mediumwith perfluorohexane, the authors could exclude that the inhibitory effectof perfluorohexane was caused by binding or sequestering limited amounts of Lipopolysaccharide. Conclusion: Taken together, our results demonstrate an interference of perfluorohexane with the expression of the procoagulant protein TF on monocytes and AMs as well as with the release of proinflammatory cytokines by MBCs. These effects may contribute to the protective role of liquid ventilation with perfluorocarbons in injuries associated with local activation of inflammatory processes.

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Documento generato il 08/08/20 alle ore 20:17:47