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Titolo:
Comparison of immunohistochemistry and silver stain for the diagnosis of pediatric Helicobacter pylori infection in urease-negative gastric biopsies
Autore:
Eshun, JK; Black, DD; Casteel, HB; Horn, H; Beavers-May, T; Jetton, CA; Parham, DM;
Indirizzi:
Arkansas Childrens Hosp, Dept Pediat Pathol, Little Rock, AR 72202 USA Arkansas Childrens Hosp Little Rock AR USA 72202 ittle Rock, AR 72202 USA Univ Tennessee, Hlth Sci Ctr, LeBonheur Childrens Hosp, Dept Pediat,Div Pediat Gastroenterol Hepatol & Nu, Memphis, TN 38103 USA Univ Tennessee Memphis TN USA 38103 l Hepatol & Nu, Memphis, TN 38103 USA Pediat Gastroenterol Associates, Little Rock, AR 72205 USA Pediat Gastroenterol Associates Little Rock AR USA 72205 ck, AR 72205 USA Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA
Titolo Testata:
PEDIATRIC AND DEVELOPMENTAL PATHOLOGY
fascicolo: 1, volume: 4, anno: 2001,
pagine: 82 - 88
SICI:
1093-5266(200101/02)4:1<82:COIASS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMULTANEOUS VISUALIZATION; CAMPYLOBACTER-PYLORI; CHILDREN; DISEASE;
Keywords:
gastritis; Helicobacter pylori; immunostain; urease test;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Parham, DM Arkansas Childrens Hosp, Dept Pathol, Slot 820,800 Marshall St,Little Rock, AR 72202 USA Arkansas Childrens Hosp Slot 820,800 Marshall StLittle Rock AR USA 72202
Citazione:
J.K. Eshun et al., "Comparison of immunohistochemistry and silver stain for the diagnosis of pediatric Helicobacter pylori infection in urease-negative gastric biopsies", PEDIATR D P, 4(1), 2001, pp. 82-88

Abstract

We compared immunohistochemical and silver stains of pediatric gastric biopsy sections for the identification of Helicobacter pylori infection with chronic inflammation and a negative urease screening test. Thirty-seven patients (age range 10 months to 21 years) whose gastric antral biopsies were negative for the rapid urease test (CLOR) but positive for lymphocytic infiltration were selected for a retrospective study. Specimens had been subjected to a rapid urease test (CLOR) and hematoxylin and eosin staining, and Dieterle silver staining and immunohistochemical staining specific for H. pylori were also performed. Twelve additional patients with urease-positive biopsies were used as controls. With Dieterle staining, 8/37 (22%) urease-negative biopsies contained organisms morphologically compatible with H. pylori, 21/37 (56%) contained organisms not compatible with H. pylori, and 8/37 (22%) were negative for organisms. Immunostaining confirmed 6/8 (75%) Dieterle-positive cases as being H. pylori, was negative in 2/8 (25%) Dieterle-positive cases, and was positive in 2/8 (25%) Dieterle-negative cases. Biopsies from 8/12 (67%) urease-positive specimens contained organisms seen withboth Dieterle and immunohistochemical stains, and 4/12 (33%) were negativewith both stains. Although both stains yielded comparable results with H. pylori-positive biopsies, Dieterle staining was potentially confusing because of nonspecific staining of other organisms. A significant proportion of (CLOR)-negative biopsies was positive for H. pylori with special stains. Wetherefore recommend the use of immunohistochemical staining rather than silver staining in the evaluation of urease-negative gastric biopsies demonstrating chronic inflammation in children.

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Documento generato il 28/02/20 alle ore 13:10:57