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Titolo:
Concentration-dependent effects of protein phosphatase (PP) inhibitors implicate PP1 and PP2A in different stages of memory formation
Autore:
Bennett, PC; Zhao, WQ; Ng, KT;
Indirizzi:
Monash Univ, Dept Psychol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 chol, Clayton, Vic 3168, Australia
Titolo Testata:
NEUROBIOLOGY OF LEARNING AND MEMORY
fascicolo: 1, volume: 75, anno: 2001,
pagine: 91 - 110
SICI:
1074-7427(200101)75:1<91:CEOPP(>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; DAY-OLD CHICKS; KINASE-C SUBSTRATE; OKADAIC ACID; RAT HIPPOCAMPUS; MUTANT MICE; B-50 GAP-43; LATE-PHASE; PHOSPHORYLATION; BRAIN;
Keywords:
calyculin A; day-old chick; dephosphorylation; learning; memory; okadaic acid; passive-avoidance learning; phosphatases; phosphorylation; synaptic plasticity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Ng, KT Monash Univ, Dept Psychol, Wellington Rd, Clayton, Vic 3168, Australia Monash Univ Wellington Rd Clayton Vic Australia 3168 168, Australia
Citazione:
P.C. Bennett et al., "Concentration-dependent effects of protein phosphatase (PP) inhibitors implicate PP1 and PP2A in different stages of memory formation", NEUROBIOL L, 75(1), 2001, pp. 91-110

Abstract

Numerous studies have demonstrated roles for protein phosphorylation and for specific kinases in memory formation; however, a role for specific protein phosphatases has not been established. Previous studies using pharmacobehavioral methods to implicate protein phosphatase activity in memory formation have been unable to discriminate between protein phosphatases 1 (PP1) and 2A (PP2A), as available cell-permeable agents generally inhibit both enzyme classes. To address this difficulty the present study exploited differences in the potency of the selective phosphatase inhibitor, okadaic acid, toward PP1 and PP2A. Within the context of a temporally precise animal modelof memory, developed using the day-old chick (Gallus domesticus), acute administration of various concentrations of okadaic acid was found to disrupttwo temporally distinct stages of memory formation. When administered bilaterally into an area of the chick brain implicated in memory formation, concentrations of okadaic acid known to selectively inhibit PP2A in vitro disrupted memory from 50 min posttraining. Higher concentrations, reported to inhibit both PP2A and PP1 in vitro, produced significant retention deficits from 20 min posttraining. Identical temporally specific effects were also obtained by varying the concentration and time of administration of calyculin A, a phosphatase inhibitor with equal potency toward both enzyme classes. Hence, different phosphatase enzymes may contribute to different stages ofthe enzymatic cascade believed to underlie memory formation, (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:52:29