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Titolo:
How the Pseudomonas aeruginosa ExoS toxin downregulates Rac
Autore:
Wurtele, M; Wolf, E; Pederson, KJ; Buchwald, G; Ahmadian, MR; Barbieri, JT; Wittinghofer, A;
Indirizzi:
Max Planck Inst Mol Physiol, Abt Strukturelle Biol, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Dortmund Germany D-44227 7 Dortmund, Germany Med Coll Wisconsin, Milwaukee, WI 53226 USA Med Coll Wisconsin Milwaukee WI USA 53226 consin, Milwaukee, WI 53226 USA
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 1, volume: 8, anno: 2001,
pagine: 23 - 26
SICI:
1072-8368(200101)8:1<23:HTPAET>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTPASE-ACTIVATING PROTEIN; TRANSITION-STATE ANALOG; ALUMINUM FLUORIDE; RHO-GTPASES; COMPLEX; HYDROLYSIS; CDC42; ARGININE; ERRORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Wittinghofer, A Max Planck Inst Mol Physiol, Abt Strukturelle Biol, Otto Hahn Str 11, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Otto HahnStr 11 Dortmund Germany D-44227
Citazione:
M. Wurtele et al., "How the Pseudomonas aeruginosa ExoS toxin downregulates Rac", NAT ST BIOL, 8(1), 2001, pp. 23-26

Abstract

Pseudomonas aeruginosa is an opportunistic bacterial pathogen. One of its major toxins, ExoS, is translocated into eukaryotic cells by a type III secretion pathway. ExoS is a dual function enzyme that affects two different pas-related GTP binding proteins. The C-terminus inactivates pas through ADPribosylation, while the N-terminus inactivates Rho proteins through its GTPase activating protein (GAP) activity. Here we have determined the three-dimensional structure of a complex between pac and the GAP domain of ExoS inthe presence of GDP and AIF(3). Composed of similar to 130 residues, this ExoS domain is the smallest GAP hitherto described. The GAP domain of ExoS is an all-helical protein with no obvious structural homology, and thus no recognizable evolutionary relationship, with the eukaryotic RhoGAP or RasGAP fold. Similar to other GAPs, ExoS downregulates pac using an arginine finger to stabilize the transition state of the GTPase reaction, but the details of the ExoS-Rac interaction are unique. Considering the intrinsic resistance of P. aeruginosa to antibiotics, this might open up a new avenue towards blocking its pathogenicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 23:05:22