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Titolo:
Widespread occurrence of spliceosomal introns in the rDNA genes of ascomycetes
Autore:
Bhattacharya, D; Lutzoni, F; Reeb, V; Simon, D; Nason, J; Fernandez, F;
Indirizzi:
Univ Iowa, Dept Biol Sci, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 , Dept Biol Sci, Iowa City, IA 52242 USA Field Museum Nat Hist, Dept Bot, Chicago, IL 60605 USA Field Museum Nat Hist Chicago IL USA 60605 ept Bot, Chicago, IL 60605 USA Univ Illinois, Dept Biol Sci, Chicago, IL 60680 USA Univ Illinois ChicagoIL USA 60680 , Dept Biol Sci, Chicago, IL 60680 USA
Titolo Testata:
MOLECULAR BIOLOGY AND EVOLUTION
fascicolo: 12, volume: 17, anno: 2000,
pagine: 1971 - 1984
SICI:
0737-4038(200012)17:12<1971:WOOSII>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL NUCLEAR-RNA; GROUP-I INTRON; RIBOSOMAL-RNA; SSU RDNA; CODING REGIONS; EVOLUTION; SITES; YEAST; PHYLOGENY; SEQUENCES;
Keywords:
ascomycetes; group I introns; lichen symbiosis; proto-splice site; rDNA; reverse-splicing; spliceosomal intron origin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Bhattacharya, D Univ Iowa, Dept Biol Sci, 239 Biol Bldg, Iowa City, IA 52242 USA Univ Iowa 239 Biol Bldg Iowa City IA USA 52242 A 52242 USA
Citazione:
D. Bhattacharya et al., "Widespread occurrence of spliceosomal introns in the rDNA genes of ascomycetes", MOL BIOL EV, 17(12), 2000, pp. 1971-1984

Abstract

Spliceosomal (pre-mRNA) introns have previously been found in eukaryotic protein-coding genes, in the small nuclear RNAs of some fungi, and in the small- and large-subunit ribosomal DNA genes of a limited number of ascomycetes. How the majority of these introns originate remains an open question because few proven cases of recent and pervasive intron origin have been documented. We report here the widespread occurrence of spliceosomal introns (69 introns at 27 different sites) in the small- and large-subunit nuclear-encoded rDNA of lichen-forming and free-living members of the Ascomycota. Ouranalyses suggest that these spliceosomal introns are of relatively recent origin, i.e., within the Euascomycetes, and have arisen through aberrant reverse-splicing tin trans) of free pre-mRNA introns into rRNAs. The spliceosome itself, and not an external agent (e.g., transposable elements, group II introns), may have given rise to these introns. A nonrandom sequence pattern was found at sites flanking the rRNA spliceosomal introns. This pattern(AG-intron-G) closely resembles the proto-splice site (MAG-intron-R) postulated for intron insertions in pre-mRNA genes. The clustered positions of spliceosomal introns on secondary structures suggest that particular rRNA regions are preferred sites for insertion through reverse-splicing.

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Documento generato il 04/04/20 alle ore 02:21:44