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Titolo:
Analysis of neural stem cells by flow cytometry: cellular differentiation modifies patterns of MHC expression
Autore:
McLaren, FH; Svendsen, CN; Van der Meide, P; Joly, E;
Indirizzi:
Babraham Inst, Mol Immunol Programme, Lab Funct Immunogenet, Cambridge CB24AT, England Babraham Inst Cambridge England CB2 4AT genet, Cambridge CB24AT, England Univ Cambridge, Cambridge Ctr Brain Repair, Cambridge CB2 2P9, England Univ Cambridge Cambridge England CB2 2P9 air, Cambridge CB2 2P9, England Univ Utrecht, Cytokine Biol Unit, CLAI, Utrecht, Netherlands Univ UtrechtUtrecht Netherlands Biol Unit, CLAI, Utrecht, Netherlands
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 1-2, volume: 112, anno: 2001,
pagine: 35 - 46
SICI:
0165-5728(20010101)112:1-2<35:AONSCB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NECROSIS-FACTOR-ALPHA; CLASS-I-ANTIGENS; MONOCLONAL-ANTIBODIES; INTERFERON-GAMMA; GENE-EXPRESSION; MULTIPLE-SCLEROSIS; IMMUNE-RESPONSE; BETA-TUBULIN; BRAIN;
Keywords:
neural stem cell; MHC; neurosphere;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Joly, E CHU Purpan, UPCM, CNRS, Unite 1590, F-31300 Toulouse, France CHU Purpan Toulouse France F-31300 590, F-31300 Toulouse, France
Citazione:
F.H. McLaren et al., "Analysis of neural stem cells by flow cytometry: cellular differentiation modifies patterns of MHC expression", J NEUROIMM, 112(1-2), 2001, pp. 35-46

Abstract

Neural stem cells are currently considered very hopeful candidates for cell replacement therapy in neurodegenerative pathologies such as Parkinson's disease. Here we show that different cell types derived from neurospheres amplified in vitro can be identified by FACS analysis relying solely on physical parameters (FSC/SSC) or autofluorescence. Additionally, after treatment with a panel of inflammatory cytokines, neurospheres and their differentiated progeny were shown to express MHC antigens which could potentially cause transplant rejection. Astrocytes expressed the highest levels of MHC. Hence removing such cells prior to transplantation could potentially optimisetransplant survival. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:32:21