Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Two rat brain Staufen isoforms differentially bind RNA
Autore:
Monshausen, M; Putz, U; Rehbein, M; Schweizer, M; DesGroseillers, L; Kuhl, D; Richter, D; Kindler, S;
Indirizzi:
Univ Hamburg, Inst Cell Biochem & Clin Neurobiol, D-20246 Hamburg, GermanyUniv Hamburg Hamburg Germany D-20246 Neurobiol, D-20246 Hamburg, Germany Univ Hamburg, Inst Neurosignaltransduct, Ctr Mol Neurobiol, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 Neurobiol, D-20246 Hamburg, Germany Univ Montreal, Dept Biochem, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 em, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 76, anno: 2001,
pagine: 155 - 165
SICI:
0022-3042(200101)76:1<155:TRBSID>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCAL PROTEIN-SYNTHESIS; OSKAR MESSENGER-RNA; ROUGH ENDOPLASMIC-RETICULUM; HIPPOCAMPAL-NEURONS; DROSOPHILA OOCYTE; DENDRITIC LOCALIZATION; MAMMALIAN STAUFEN; XENOPUS OOCYTES; GERM PLASM; MICROTUBULES;
Keywords:
cytoskeleton; dendritic RNAs; extrasomatic localization; microtubule-associated protein; RNA-binding protein; trans-acting factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Kindler, S Univ Hamburg, Inst Cell Biochem & Clin Neurobiol, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 rg, Germany
Citazione:
M. Monshausen et al., "Two rat brain Staufen isoforms differentially bind RNA", J NEUROCHEM, 76(1), 2001, pp. 155-165

Abstract

In neurones, a limited number of mRNAs is found in dendrites, including transcripts encoding the microtubule-associated protein 2 (MAP2). Recently, we identified a cis-acting dendritic targeting element (DTE) in MAP2 mRNAs. Here we used the yeast tri-hybrid system to identify potential trans-actingRNA-binding factors of the DTE. A cDNA clone was isolated that encodes a member of a mammalian protein family that is highly homologous to the Drosophila RNA-binding protein Staufen. Mammalian Staufen appears to be expressedin most tissues and brain areas. Two distinct rat brain Staufen isoforms, rStau+I6 and rStau-I6, are encoded by alternatively spliced mRNAs, Both isoforms contain four double-stranded RNA-binding domains (dsRBD). In the larger rStau+I6 isoform, six additional amino acids are inserted in the second dsRBD, Although both isoforms interacted with the MAP2-DTE and various additional RNA fragments in an in vitro north-western assay, rStau-I6 exhibiteda stronger signal of bound radioactively labelled RNAs as compared with rStau+I6. Using an antibody directed against mammalian Staufen, the protein was detected in somata and dendrites of neurones of the adult rat hippocampus and cerebral cortex. Ultrastructural studies revealed that in dendrites, rat Staufen accumulates along microtubules. Thus in neurones, rat Staufen may serve to link RNAs to the dendritic microtubular cytoskeleton and may thereby regulate their subcellular localization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 00:21:23