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Titolo:
Characterization of 3-[I-123]iodo-L-alpha-methyl tyrosine transport in astrocytes of neonatal rats
Autore:
Kopka, K; Riemann, B; Friedrich, M; Winters, S; Halfter, H; Weckesser, M; Stogbauer, F; Ringelstein, EB; Schober, O;
Indirizzi:
Univ Munster, Dept Nucl Med, D-48129 Munster, Germany Univ Munster Munster Germany D-48129 Nucl Med, D-48129 Munster, Germany Univ Munster, Dept Neurol, D-48129 Munster, Germany Univ Munster MunsterGermany D-48129 pt Neurol, D-48129 Munster, Germany
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 76, anno: 2001,
pagine: 97 - 104
SICI:
0022-3042(200101)76:1<97:CO3TTI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; BLOOD-BRAIN-BARRIER; L-3-IODO-ALPHA-METHYL TYROSINE; CEREBRAL GLIOMAS; ANIMAL-CELLS; TUMOR UPTAKE; L-ARGININE; IN-VIVO; SPECT; EXPRESSION;
Keywords:
C6 glioma cells; I-123-IMT; 3-[I-123]iodo-L-alpha-methyl tyrosine; LAT-1; neonatal astrocytes; system L;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kopka, K Univ Munster, Dept Nucl Med, Albert Schweitzer Str 33, D-48129 Munster, Germany Univ Munster Albert Schweitzer Str 33 Munster Germany D-48129 ny
Citazione:
K. Kopka et al., "Characterization of 3-[I-123]iodo-L-alpha-methyl tyrosine transport in astrocytes of neonatal rats", J NEUROCHEM, 76(1), 2001, pp. 97-104

Abstract

3-[I-123]Iodo-L-alpha -methyl tyrosine (I-123-IMT) is used for diagnosis and monitoring of brain tumours by means of single-photon emission tomography. As recently shown, I-123-IMT is predominantly mediated into rat C6 glioma cells by sodium-independent system L for large neutral amino acids. Untilnow, I-123-IMT transport in non-neoplastic glial cells has not been examined. Therefore, the aim of this study was to examine the cellular pathways and precise transport kinetics of I-123-IMT uptake into astrocytes of neonatal rats. In particular sodium-independent I-123-IMT transport into neonatalastrocytes was compared with sodium-independent I-123-IMT uptake into neoplastic rat C6 glioma cells. Competitive inhibition experiments showed that I-123-IMT is exclusively transported via sodium-independent system L into the neonatal astrocytes (92%). Kinetic analysis of sodium-independent I-123-IMT uptake into neonatal astrocytes and into C6 glioma cells revealed apparent Michaelis constants K-M= 13.9 +/- 0.5 muM and K-M = 33.9 +/- 4.1 muM, respectively, which are in the same range of K-M values as those recently determined for amino acid transport into neoplastic and non-neoplastic glial cells. Indeed, the K-M values in the micromolar range correspond to the expression of the LAT-1 subunit of system L both in the neonatal astrocytes and in C6 glioma cells. However, sodium-independent maximum transport velocities (V-max) differed significantly between neonatal astrocytes and C6 glioma cells (11.1 +/- 0.3 and 39.9 +/- 3.3 nmol/mg protein/10 min, respectively).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 11:54:02