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Titolo:
Suppression of interferon-induced antiviral activity in cells expressing hepatitis C virus proteins
Autore:
Aizaki, H; Saito, S; Ogino, T; Miyajima, N; Harada, T; Matsuura, Y; Miyamura, T; Kohase, M;
Indirizzi:
Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Musashimurayama, Tokyo 2080011, Japan Natl Inst Infect Dis Musashimurayama Tokyo Japan 2080011 o 2080011, Japan NIID, Dept Virol 2, Tokyo 1628640, Japan NIID Tokyo Japan 1628640NIID, Dept Virol 2, Tokyo 1628640, Japan Univ Giessen, Inst Virol FB10, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 irol FB10, D-35392 Giessen, Germany
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 12, volume: 20, anno: 2000,
pagine: 1111 - 1120
SICI:
1079-9907(200012)20:12<1111:SOIAAI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVITY-DETERMINING REGION; NONSTRUCTURAL 5A PROTEIN; TUMOR NECROSIS FACTOR; AMINO-ACID SUBSTITUTIONS; CORE PROTEIN; NS5A PROTEIN; GENOTYPE 1B; GROWTH-FACTOR; ALFA THERAPY; MUTATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kohase, M Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, 4-7-1 Gakuen, Musashimurayama, Tokyo 2080011, Japan Natl Inst Infect Dis 4-7-1 Gakuen Musashimurayama Tokyo Japan 2080011
Citazione:
H. Aizaki et al., "Suppression of interferon-induced antiviral activity in cells expressing hepatitis C virus proteins", J INTERF CY, 20(12), 2000, pp. 1111-1120

Abstract

To elucidate the mechanism of the persistent nature of hepatitis C virus (HCV) infection, we examined whether the expression of HCV proteins affect the antiviral activity of interferon (IFN). Antiviral activity of IFN in HepG2 cells expressing all HCV (type 1b) proteins was much lower than vector control (VC) HepG2 cells when encephalomyocarditis virus (EMCV) was used as a challenge virus. Lesser sensitivity to IFN was also observed in cells expressing NS3, NS4, and NS5 acid in cells expressing only NS5A. In contrast, HepG2 cells expressing core, E1, E2, NS2, and NS3 proteins were equally sensitive to IFN as VC cells. We then tested the antiviral activity by IFN in two human amnion-derived FL cell lines expressing NS5A from two different clones, one with an intact sequence of IFN sensitivity-determining region (ISDR) and the other with a mutated ISDR sequence. They were almost equally insensitive to IFN treatment when EMCV was challenged. HCV thus has functional protein(s), possibly NS5A, to suppress IFN-induced antiviral activity and plays an important role in virus-cell interaction and regulation of viralreplication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:30:28