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Titolo:
An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK
Autore:
Zochowski, WJ; Palmer, MF; Coleman, TJ;
Indirizzi:
Cty Hosp, PHLS, Leptospira Reference Unit,WHO, FAO,Collaborating Ctr Res Leptospirosis, Hereford HR1 2ER, England Cty Hosp Hereford England HR1 2ERptospirosis, Hereford HR1 2ER, England
Titolo Testata:
JOURNAL OF CLINICAL PATHOLOGY
fascicolo: 1, volume: 54, anno: 2001,
pagine: 25 - 30
SICI:
0021-9746(200101)54:1<25:AEOTCK>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; AGGLUTINATION-TEST; IMMUNOGLOBULIN-M; ELISA; SERODIAGNOSIS; DIAGNOSIS; IGM;
Keywords:
leptospirosis; enzyme linked immunosorbent assay; indirect haemagglutination assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Zochowski, WJ Cty Hosp, PHLS, Leptospira Reference Unit,WHO, FAO,Collaborating Ctr Res Leptospirosis, Hereford HR1 2ER, England Cty Hosp Hereford England HR1 2ER ereford HR1 2ER, England
Citazione:
W.J. Zochowski et al., "An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK", J CLIN PATH, 54(1), 2001, pp. 25-30

Abstract

Aims-To compare three commercial screening tests-the PanBio leptospiral IgM enzyme linked immunosorbent assay (ELISA), the Biolisa leptospiral IgM ELISA, and the indirect haemagglutination assay (IHA)-with the microscopic agglutination test (MAT) and two "in house" ELISAs-urease and horseradish peroxidase (HRP)-for the detection of leptospiral antibodies in a local UK andfire population. Method-Two hundred sera submitted for a differential diagnosis of leptospirosis were tested by all methods. A further 142 sera from patients with antibodies to toxoplasma, Epstein-Barr virus (EBV), hepatitis A virus, rheumatoid factor, Borrelia burgdorferi, Mycoplasma pneumoniae, syphilis, cytomegalovirus, and Q fever were tested for crossreactivity. Results-Compared with the MAT, sensitivity and specificity were found to be: PanBio, 90%/94%; Biolisa with sorbent, 100%/85%; and IHA, 54%/95%. Sevenof 200 trial sera gave false negative results with PanBio; 14 of 200 trialsera gave false positive results with Biolisa with sorbent, as did a further 25 of the 142 sera tested for potential crossreactivity. Two of 142 seragave crossreactions with PanBio and IHA tone each). Conclusions-The degree of false positivity seen with the Biolisa suggests that the recommended positive value of greater than or equal to 26 Eu/ml should be reassessed using pools of sera from local populations. When the cutoff value was reassessed, using a value of greater than or equal to 40 Eu/ml, a sensitivity and specificity of 96% and 94%, respectively, was achieved. Even the modified Biolisa appears to be over sensitive and to show a high degree of non-specificity. The IHA, although specific (95%), lacked sensitivity in this study. The PanBio appeared to be the most suitable as a screening test for leptospiral IgM in the UK, although it would be advisable for all positive test results to be confirmed by a different enzyme immunoassay and the MAT.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:20:28