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Titolo:
Expression cloning of human globoside synthase cDNAs - Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine: Globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase
Autore:
Okajima, T; Nakamura, Y; Uchikawa, M; Haslam, DB; Numata, S; Furukawa, K; Urano, T; Furukawa, K;
Indirizzi:
Nagoya Univ, Sch Med, Dept Biochem 2, Showa Ku, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 a Ku, Nagoya, Aichi 4660065, Japan Japanese Red Cross Cent Blood Ctr, Shibuya Ku, Tokyo 150, Japan Japanese Red Cross Cent Blood Ctr Tokyo Japan 150 a Ku, Tokyo 150, Japan Washington Univ, Sch Med, Dept Pediat, Child Hlth Res Ctr Excellence Dev Biol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 ce Dev Biol, St Louis, MO 63110 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 51, volume: 275, anno: 2000,
pagine: 40498 - 40503
SICI:
0021-9258(200012)275:51<40498:ECOHGS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; BINDING-SPECIFICITY; GENE; GALACTOSE; BETA-1,3-GALACTOSYLTRANSFERASE; FAMILY; CELLS; PK; BIOSYNTHESIS; GLYCOLIPIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Nagoya Univ, Sch Med, Dept Biochem 2, Showa Ku, 65 Tsurumai, Nagoya, Aichi4660065, Japan Nagoya Univ 65 Tsurumai Nagoya Aichi Japan 4660065 0065, Japan
Citazione:
T. Okajima et al., "Expression cloning of human globoside synthase cDNAs - Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine: Globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase", J BIOL CHEM, 275(51), 2000, pp. 40498-40503

Abstract

By using a eukaryocytic cell expression cloning system, we have isolated cDNAs of the globoside synthase (beta1,3-N-acetylgalactosaminyltransferase) gene. Mouse fibroblast L cells transfected with SV40 large T antigen and previously cloned Gb3/CD77 synthase cDNAs were co-transfected with a cDNA library prepared from mRNA from human kidney together with Forssman synthase cDNA, and Forssman antigen-positive cells were panned using an anti-Forssmanmonoclonal antibody. The isolated cDNAs contained a single open reading frame predicting a type II membrane protein with 351 amino acids. Surprisingly, the cDNA clones turned out to be identical with previously reported beta3Gal-T3, which had been cloned by sequence homology with other galactosyltransferases. Substrate specificity analysis with extracts from cDNA-transfected L cells confirmed that the gene product was actually pl,3-N-acetylgalactosaminyltransferase that specifically catalyzes the transfer of N-acetylgalactosamine onto globotriaosylceramide. Results of TLC immunostaining of neutral glycolipids from the cDNA-transfected cells also supported the identity of the newly synthesized component as globoside. The results show that glycosyltransferases apparently belonging to a single glycosyltransferase family do not necessarily catalyze reactions utilizing the same acceptor or even the same sugar donor. The globoside synthase gene was expressed in many tissues, such as heart, brain, testis, etc. We propose the designation beta 3GalNAc-T1 for the cloned globoside synthase gene.

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Documento generato il 15/01/21 alle ore 23:05:27