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Titolo:
E1A(12S)-mediated activation of the adenovirus type 12 E2 promoter dependson the histone acetyltransferase activity of p300/CBP
Autore:
Fax, P; Lehmkuhler, O; Kuhn, C; Esche, H; Brockmann, D;
Indirizzi:
Univ Essen Gesamthsch, Sch Med, Inst Mol Biol Canc Res, D-45122 Essen, Germany Univ Essen Gesamthsch Essen Germany D-45122 Res, D-45122 Essen, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 51, volume: 275, anno: 2000,
pagine: 40554 - 40560
SICI:
0021-9258(200012)275:51<40554:EAOTAT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENERAL TRANSCRIPTION FACTORS; RNA-POLYMERASE-II; COACTIVATORS P300; ONCOPROTEIN E1A; ONCOGENIC ADENOVIRUS-TYPE-12; TRANSACTIVATION FUNCTION; CBP; ACETYLATION; PROTEINS; PCAF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Fax, P Univ Essen Gesamthsch, Sch Med, Inst Mol Biol, Hufelandstr 55, D-45122 Essen, Germany Univ Essen Gesamthsch Hufelandstr 55 Essen Germany D-45122 Germany
Citazione:
P. Fax et al., "E1A(12S)-mediated activation of the adenovirus type 12 E2 promoter dependson the histone acetyltransferase activity of p300/CBP", J BIOL CHEM, 275(51), 2000, pp. 40554-40560

Abstract

Activation of the transcription unit early region 2 (E2) promoter of the oncogenic adenovirus serotype 12 (Ad12), which regulates the expression of proteins essential for viral replication, requires the assembly of a ternarycomplex consisting of cAMP response element-binding protein (CREB)-1/activating transcription factor (ATF)-1, the Ad12 12S oncogene product of early region 1A (E1A(12S)), and the co-activator p300/CBP on the E2(Ad12) cAMP response element (ES-CRE). Here we show that the active E2(Ad12) promoter is associated with acetylated histone H4 whereas an E2-CRE point-mutated promoter which is transcriptionally inactive due to its inability to assemble this ternary complex is not bound by acetylated histone H4. The histone deacetylase 1 as well as Roscovitine, which blocks the activation of the histoneacetyltransferase (HAT) activity of CBP by cyclin E-Cdk2, prevents E2(Ad12) promoter activation through E1A(12S). p300/CBP counteracts the repressivefunction of histone deacetylase 1 in a HAT domain-dependent manner whereasthe p300/CBP-associated factor PCAF failed to rescue E2(Ad12) promoter activity. E1A(12S) bound p300/CBP displays strong HAT activity. Most interestingly, E1A(12S)-mediated activation of the E2(Ad12) promoter correlates wellwith the ability of the viral protein to associate with the HAT activity of p300/CBP in vivo. Taken together these data indicate that the recruitmentof the HAT activity of p300/CBP by E1A(12S) plays an important role in E2(Ad12) promoter activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 05:58:58