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Titolo:
Transcriptional analysis of chromatin assembled with purified ACF and dNAP1 reveals that acetyl-CoA is required for preinitiation complex assembly
Autore:
Jiang, W; Nordeen, SK; Kadonaga, JT;
Indirizzi:
Univ Calif San Diego, Sect Mol Biol, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 l Biol, La Jolla, CA 92093 USA Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 Genet, La Jolla, CA 92093 USA Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Pathol, Denver, CO 80262 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 51, volume: 275, anno: 2000,
pagine: 39819 - 39822
SICI:
0021-9258(200012)275:51<39819:TAOCAW>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA POLYMERASE-II; HISTONE ACETYLTRANSFERASE COMPLEXES; SOLUBLE NUCLEAR FRACTION; DROSOPHILA EMBRYOS; FUNCTIONAL STEPS; ACTIVATION; INITIATION; EXTRACT; REINITIATION; TEMPLATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kadonaga, JT Univ Calif San Diego, Sect Mol Biol, 0347 Pacific Hall,Rm 2212B, La Jolla,CA 92093 USA Univ Calif San Diego 0347 Pacific Hall,Rm 2212B La Jolla CA USA 92093
Citazione:
W. Jiang et al., "Transcriptional analysis of chromatin assembled with purified ACF and dNAP1 reveals that acetyl-CoA is required for preinitiation complex assembly", J BIOL CHEM, 275(51), 2000, pp. 39819-39822

Abstract

To investigate the role of chromatin structure in the regulation of transcription by RNA polymerase II, we developed a chromatin transcription systemin which periodic nucleosome arrays are assembled with purified recombinant ATP-utilizing chromatin assembly and remodeling factor (ACF), purified recombinant nucleosome assembly protein 1 (dNAP1), purified native core histones, plasmid DNA, and ATP. With this chromatin, we observed robust activation of transcription with three different transcription factor sets (nuclearfactor kappaB p65 + Sp1 estrogen receptor, and Ga14-VP16) added either before or after chromatin assembly. In fact, the efficiency of activated transcription from the ACF + dNAP1-assembled chromatin was observed to be comparable with that from naked DNA templates or chromatin assembled with a crudeDrosophila extract (S190). With ACF + dNAP1-assembled chromatin, we found that transcriptional activation is dependent upon acetyl-CoA. This effect was not seen with naked DNA templates or with crude S190-assembled chromatin. We further determined that acetyl-CoA is required at the time of preinitiation complex assembly but not during assembly of the chromatin template. These findings suggest that there is at least one key acetylation event thatis needed to assemble a functional transcription preinitiation complex with a chromatin template.

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Documento generato il 22/01/20 alle ore 18:32:03