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Titolo:
Interaction of MyoD family proteins with enhancers of acetylcholine receptor subunit genes in vivo
Autore:
Liu, SH; Spinner, DS; Schmidt, MM; Danielsson, JA; Wang, SW; Schmidt, J;
Indirizzi:
SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA SUNYStony Brook Stony Brook NY USA 11794 Biol, Stony Brook, NY 11794 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 52, volume: 275, anno: 2000,
pagine: 41364 - 41368
SICI:
0021-9258(200012)275:52<41364:IOMFPW>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE-CELL CULTURES; E-BOX ELEMENTS; ELECTRICAL-ACTIVITY; MYOGENIC FACTORS; SKELETAL-MUSCLE; DENERVATION SUPERSENSITIVITY; DEPENDENT EXPRESSION; EPSILON-SUBUNIT; TRANSGENIC MICE; BINDING-SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt, J SUNY Stony Brook, Dept Biochem & Cell Biol, Life Sci Bldg,Rm 412, Stony Brook, NY 11794 USA SUNY Stony Brook Life Sci Bldg,Rm 412 Stony Brook NY USA 11794
Citazione:
S.H. Liu et al., "Interaction of MyoD family proteins with enhancers of acetylcholine receptor subunit genes in vivo", J BIOL CHEM, 275(52), 2000, pp. 41364-41368

Abstract

The myogenic determination factors (MDFs) are transcriptional activators that target E boxes in many muscle-specific promoters, including those of the genes coding for the subunits of the acetylcholine receptor. It is not known, however, if in vivo a given E box in a transcriptionally active gene is occupied, either uniquely by one MDF or randomly by all MDFs. We have analyzed expression of MDF and acetylcholine receptor subunits in cultured mouse muscle cells and, using chromatin immunoprecipitation, have determined which individual MDFs reside at promoters of several receptor subunit genes. We find that before fusion, C2C12 cells express myf-5, MyoD, and myogenin,all of which take up residence at promoters of all subunits except epsilon. At this stage, herculin is present in limited amounts and is detected mainly at the gamma and delta subunit genes. On myotube formation, herculin reaches high levels; concomitantly, the epsilon subunit gene becomes a commonMDF target and beans to be expressed, In general, any MDF protein that is expressed also is present on transcriptionally active receptor genes; transcriptional activity of target genes correlates with occupancy by MDF, in particular, herculin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 21:15:43