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Titolo:
Flow cytometric analysis of inflammatory markers in KCS: 6-month treatmentwith topical cyclosporin A
Autore:
Brignole, F; Pisella, PJ; De Saint Jean, M; Goldschild, M; Goguel, A; Baudouin, C;
Indirizzi:
Univ Paris 05, APHP, Ambroise Pare Hosp, Dept Ophthalmol, F-92104 Boulogne, France Univ Paris 05 Boulogne France F-92104 hthalmol, F-92104 Boulogne, France Univ Paris 05, APHP, Ambroise Pare Hosp, Lab Immunohematol, F-92104 Boulogne, France Univ Paris 05 Boulogne France F-92104 ohematol, F-92104 Boulogne, France
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 1, volume: 42, anno: 2001,
pagine: 90 - 95
SICI:
0146-0404(200101)42:1<90:FCAOIM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CONJUNCTIVAL EPITHELIUM; KERATOCONJUNCTIVITIS SICCA; SJOGRENS-SYNDROME; OCULAR SURFACE; ANTIGEN EXPRESSION; GENE-EXPRESSION; LACRIMAL GLAND; LIGAND; CELLS; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Baudouin, C Univ Paris 05, APHP, Ambroise Pare Hosp, Dept Ophthalmol, 9 Ave Charles deGaulle, F-92104 Boulogne, France Univ Paris 05 9 Ave Charles deGaulle Boulogne France F-92104
Citazione:
F. Brignole et al., "Flow cytometric analysis of inflammatory markers in KCS: 6-month treatmentwith topical cyclosporin A", INV OPHTH V, 42(1), 2001, pp. 90-95

Abstract

PURPOSE. Immune-based inflammation has been observed as a common mechanismof keratoconjunctivitis sicca (KCS). In KCS-affected eves, upregulated expression of HLA DR and various immune- or apoptosis-related markers by conjunctival epithelial cells has been demonstrated in an earlier study, by a technique of flow cytometry in impression cytology (IC) specimens. The purpose of this study was to monitor the effects of topical cyclosporin A on the expression of these markers throughout a 6-month period of treatment. METHODS, Patients with moderate to severe KCS included in a large Europeanmulticenter clinical trial (Cyclosporin Dry Eye Study, Allergan, Irvine, CA) underwent collection of IC specimens at baseline, month 3, and month 6. For 6 months, they randomly received 0.05% or 0.1% cyclosporin A of vehicle. Specimens were processed and analyzed in a masked manner by flow cytometry, using monoclonal antibodies directed to HLA DR, CD40, CD40 ligand, Fas, and the apoptotic marker APO2.7. Percentages of positive cells were calculated and levels of expression quantified after conversion into standardized units of fluorescence. RESULTS. One hundred fifty-eight patients had at least two IC specimens available for flow cytometry analysis. HLA DR expression, both in percentage of positive cells and level of expression, was highly significantly reducedafter 0.05% and 0.1% cyclosporin A treatment at months 3 and 6 compared with baseline values, whereas vehicle did not induce any change in HLA DR expression over time. The 0.05% and 0.1% cyclosporin emulsions were significantly more effective than the vehicle in reducing HLA DR at months 3 and 6 (0.05%), and at month 6 (01%). CD40 expression was significantly reduced at month 3 and partially at month 6, compared with baseline, with no reduction in patients who received the vehicle. CD40 ligand expression also decreasedat months 3 and 6 in patients taking both concentrations of cyclosporin A. APO2.7 expression was significantly increased in all three groups, whereaspercentage of Fas-positive cells decreased only in patients treated with 0.05% cyclosporin A at months 3 and 6. CONCLUSIONS. Flow cytometry provided an objective technique to monitor theeffects of topical cyclosporin A on immune- and apoptosis-related markers in the conjunctival epithelium of patients with KCS enrolled in a large multicenter trial. Topical cyclosporin A strikingly reduced HLA DR and to a lesser extent, ether inflammatory and apoptotic markers, whereas the vehicle,used as a control tear substitute, had almost no effect. This study confirms that cyclosporin A may be efficient in reducing conjunctival inflammation in moderate to severe RCS and is consistent with clinical results in thisindication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:02:18