Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The influence of stromal contraction in a wound model system on corneal epithelial stratification
Autore:
Taliana, L; Evans, MDM; Dimitrijevich, SD; Steele, JG;
Indirizzi:
Univ New S Wales, Sydney CSIRO Div Mol Sci, Cooperat Res Ctr Eye Res & Technol, N Ryde, NSW, Australia Univ New S Wales N Ryde NSW Australia & Technol, N Ryde, NSW, Australia Univ N Texas, Hlth Sci Ctr, Dept Mol Biol & Immunol, Ft Worth, TX USA UnivN Texas Ft Worth TX USA , Dept Mol Biol & Immunol, Ft Worth, TX USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 1, volume: 42, anno: 2001,
pagine: 81 - 89
SICI:
0146-0404(200101)42:1<81:TIOSCI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANE; RADIAL KERATOTOMY; IMMUNOCHEMICAL CHARACTERIZATION; DESMOSOMAL PLAQUE; STEM-CELLS; IN-VITRO; EXPRESSION; INTEGRINS; HEMIDESMOSOMES; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Taliana, L CUNY Mt Sinai Sch Med, Dept Ophthalmol, Box 1183,1 Gustave LevyPl, New York, NY 10029 USA CUNY Mt Sinai Sch Med Box 1183,1 Gustave Levy Pl New York NY USA 10029
Citazione:
L. Taliana et al., "The influence of stromal contraction in a wound model system on corneal epithelial stratification", INV OPHTH V, 42(1), 2001, pp. 81-89

Abstract

PURPOSE. The healing process of some corneal wounds involves closure by stromal contraction and the renewal of the stratified epithelium. In wound gape injury such stromal contraction occurs with epithelial stratification. In previous in vitro studies of noncontracted and contracted corneal fibroblast-seeded collagen gels (FSCGs) it was shown that initiation of wound contraction by the myofibroblast phenotype (present within the wounded stroma) was dependent on vitronectin and/or fibronectin. This study considers one aspect of the epithelial-stromal interaction that occurs during wounding. The stratification of corneal epithelial cells on noncontracted and contracted corneal FSCGs was compared. METHODS. Dissociated bovine corneal epithelial cells were seeded on noncontracted and contracted corneal FSCGs, and these assemblies were cultured for 7 days. The epithelium that formed was evaluated using laser confocal microscopy and immunohistochemical markers directed against cytokeratin 3, desmoplakin I and II, integrin ord subunit, laminin, and collagen VII. The characteristics of the epithelium were compared with stromal carriers comprised of dissociated bovine corneal epithelial cells seeded on intact stroma and basement membrane (stromal carrier biopsies). RESULTS. The stratified epithelium that developed on contracted corneal fibroblast-seeded collagen gels was similar to that formed on stromal carriers, whereas nonstratified epithelium formed on noncontracted FSCGs. CONCLUSIONS. These studies showed that the contracted state of fibroblast-seeded gels enhanced the development of a well-organized, stratified corneal epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:52:21