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Titolo:
Carbonic anhydrase localization in charophycean green algae: Ecological and evolutionary significance
Autore:
Arancibia-Avila, P; Coleman, JR; Russin, WA; Graham, JM; Graham, LE;
Indirizzi:
Univ Wisconsin, Dept Bot, Madison, WI 53706 USA Univ Wisconsin Madison WIUSA 53706 nsin, Dept Bot, Madison, WI 53706 USA Univ Bio Bio, Dept Ciencias Basicas, Chillan, Chile Univ Bio Bio ChillanChile o Bio, Dept Ciencias Basicas, Chillan, Chile Univ Toronto, Dept Bot, Toronto, ON M5S 3B2, Canada Univ Toronto Toronto ON Canada M5S 3B2 t Bot, Toronto, ON M5S 3B2, Canada Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 t Plant Pathol, Madison, WI 53706 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF PLANT SCIENCES
fascicolo: 1, volume: 162, anno: 2001,
pagine: 127 - 135
SICI:
1058-5893(200101)162:1<127:CALICG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLAMYDOMONAS-REINHARDTII; CHARA-CORALLINA; CO2-CONCENTRATING MECHANISMS; PHOTOSYNTHETIC UTILIZATION; ACIDIC CONDITIONS; EUKARYOTIC ALGAE; EXOGENOUS HCO3; IDENTIFICATION; CHLOROPLAST; DIVERSITY;
Keywords:
carbonic anhydrase; Chara; charophyceans; Chlamydomonas; green algae; Mougeotia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Graham, LE Univ Wisconsin, Dept Bot, Madison, WI 53706 USA Univ WisconsinMadison WI USA 53706 Bot, Madison, WI 53706 USA
Citazione:
P. Arancibia-Avila et al., "Carbonic anhydrase localization in charophycean green algae: Ecological and evolutionary significance", INT J PL SC, 162(1), 2001, pp. 127-135

Abstract

Two carbonic anhydrase (CA) antibodies, one to the primarily periplasmic alpha-type enzyme of the chlorophycean Chlamydomonas and another to pea (Pisum sativum) beta-type CA, which is primarily located in the chloroplast stroma, were applied to the charophycean green alga Mougeotia sp., shoot cellsof the later divergent charophycean, Chara zeylanica, and control Chlamydomonas cells and pea leaf tissues. Immunolocalizations were quantitatively assessed at the ultrastructural level; nonimmune labeling was negligible in all cases. The Chlamydomonas antibody intensely labeled inner cell wall, periplasmic region, and peripheral cytoplasm of Chlamydomonas, Mougeotia, andChara. The concentration of periplasmic label did not significantly differamong these green algae. This is the first immunolocalization evidence foroccurrence of a periplasmic CA in Chara. Chloroplasts of Mougeotia, Chara,and pea were also labeled by the Chlamydomonas antibody, suggesting that plants inherited a plastid thylakoid-based, alpha-type CA from charophycean ancestors. The pea antibody labeled Chara chloroplasts but not those of Chlamydomonas or Mougeotia. The concentration of pea antibody stromal label did not significantly differ between pea and Chara. This is the first immunolocalization evidence for occurrence of a higher-plant, beta-type stromal CAin green algal plastids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:15:45