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Titolo:
Calmodulin and its role in the secretory apparatus of the desmid Closterium
Autore:
Linde, J; Morse, L; Domozych, DS;
Indirizzi:
Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA Skidmore Coll Saratoga Springs NY USA 12866 aratoga Springs, NY 12866 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF PLANT SCIENCES
fascicolo: 1, volume: 162, anno: 2001,
pagine: 15 - 27
SICI:
1058-5893(200101)162:1<15:CAIRIT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN-ALGA CLOSTERIUM; ENDOPLASMIC-RETICULUM; MONOCLONAL-ANTIBODIES; CYCLOPIAZONIC ACID; GOLGI-APPARATUS; TIP GROWTH; CELL-WALL; CALCIUM; MICRASTERIAS; EXOCYTOSIS;
Keywords:
Closterium; calmodulin; Golgi; W7; W13; TFP; TMB-8;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Domozych, DS Skidmore Coll, Dept Biol, Saratoga Springs, NY 12866 USA Skidmore Coll Saratoga Springs NY USA 12866 gs, NY 12866 USA
Citazione:
J. Linde et al., "Calmodulin and its role in the secretory apparatus of the desmid Closterium", INT J PL SC, 162(1), 2001, pp. 15-27

Abstract

Closterium acerosum is a large bipolar desmid that has an extensive secretory apparatus. Two major secretory complexes that are produced by this green alga are a glucuronic acid-rich mucilage that is key in the gliding mechanism and a cell wall consisting of multiple polysaccharide and protein components. These substances are synthesized and processed for secretion through an elaborate endomembrane system and are transported around the cell and to the cell surface via actin-mediated cyclosis. In this study, an analysisof the role of calmodulin with regard to the secretory apparatus was conducted. Whole-cell extracts and Western blot analysis show the existence of calmodulin. Immunogold labeling reveals calmodulin in the peripheral cytoplasm near the plasmamembrane and cell wall region. Calmodulin antagonists cause significant disruption to the morphology of the Golgi apparatus. On application of the antagonists W7, W13, TMB-8, and TFP, the Golgi body exhibitsreduction in the number of cisternae per stack, significant curling of cisternae, and formation of unusual multiperforate vesicles. In addition, there is alteration of the cytoplasmic streaming network and limited disturbance to the peripheral vacuole system. Immunogold labeling with an antibody specific to the mucilage shows that mucilage processing is still apparent in the altered Golgi bodies and remnants after treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:30:23