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Titolo:
Sperm-mediated transformation of the honey bee, Apis mellifera
Autore:
Robinson, KO; Ferguson, HJ; Cobey, S; Vaessin, H; Smith, BH;
Indirizzi:
Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 ept Entomol, Columbus, OH 43210 USA Ohio State Univ, Mol Cellular & Dev Biol Program, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 iol Program, Columbus, OH 43210 USA Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 t Mol Genet, Columbus, OH 43210 USA Ohio State Univ, Neurobiotechnol Ctr, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 technol Ctr, Columbus, OH 43210 USA
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 6, volume: 9, anno: 2000,
pagine: 625 - 634
SICI:
0962-1075(200012)9:6<625:STOTHB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM-LINE TRANSFORMATION; YELLOW-FEVER MOSQUITO; SILKWORM BOMBYX-MORI; EXOGENOUS DNA; CAENORHABDITIS-ELEGANS; GENE-TRANSFER; TRANSPOSABLE ELEMENT; HERMES ELEMENT; AEDES-AEGYPTI; LEARNING-PERFORMANCE;
Keywords:
honey bee; sperm; transformation; GFP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, BH Ohio State Univ, Dept Entomol, 1735 Neil Ave, Columbus, OH 43210USA Ohio State Univ 1735 Neil Ave Columbus OH USA 43210 OH 43210 USA
Citazione:
K.O. Robinson et al., "Sperm-mediated transformation of the honey bee, Apis mellifera", INSEC MOL B, 9(6), 2000, pp. 625-634

Abstract

Our primary objective was to identify techniques to transform the genome of the honey bee (Apis mellifera) with foreign DNA constructs, The strategy we adopted was to linearize foreign DNA and introduce it with sperm during the instrumental insemination of virgin queen honey bees. We analysed extracts from larvae within the same cohort and isolated the predicted fragment by means of PCR amplification of genomic DNA. larvae that carried the construct also expressed the introduced DNA, We propagated several transgenic lines for up to three generations, which demonstrates its heritability. Once carried by a queen, the construct can be detected In that queen's larvae over several months. However, there was no evidence of integration of the construct, at least as determined by genomic Southern analysis. Nevertheless, this demonstrates the general viability of the technique for introduction of DNA, and it should be augmented by further use of transposable elements that enhance integration.

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Documento generato il 13/07/20 alle ore 11:08:07