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Titolo:
Lipo prostaglandin E1 reduces the production of CXC chemokines in endotoxin-induced rat liver injury
Autore:
Ohira, H; Suzuki, T; Shishido, S; Tojo, J; Miyata, M; Obara, K; Kasukawa, R;
Indirizzi:
Fukushima Med Univ, Sch Med, Dept Internal Med 2, Fukushima 9601295, JapanFukushima Med Univ Fukushima Japan 9601295 d 2, Fukushima 9601295, Japan
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 1, volume: 19, anno: 2001,
pagine: 74 - 84
SICI:
1386-6346(200101)19:1<74:LPERTP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; INDUCED NEUTROPHIL CHEMOATTRACTANT; MESSENGER-RNA EXPRESSION; MACROPHAGE INFLAMMATORY PROTEIN-2; KUPFFER CELLS; ISCHEMIA/REPERFUSION INJURY; HEPATIC-FAILURE; FACTOR-ALPHA; RELEASE; REPERFUSION;
Keywords:
endotoxin; macrophage inflammatory protein-2; cytokine-induced neutrophil chemoattractant; neutrophil; prostaglandin E1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Ohira, H Fukushima Med Univ, Sch Med, Dept Internal Med 2, 1-Hikarigaoka, Fukushima9601295, Japan Fukushima Med Univ 1-Hikarigaoka Fukushima Japan 9601295 , Japan
Citazione:
H. Ohira et al., "Lipo prostaglandin E1 reduces the production of CXC chemokines in endotoxin-induced rat liver injury", HEPATOL RES, 19(1), 2001, pp. 74-84

Abstract

The present study attempted to assess the effect of prostaglandin El (PGE1) incorporated into lipid microspheres (Lipo PGE1) on chemokine production in endotoxin-induced rat liver injury. Male Wistar rats weighing 200-250 g were injected with 2 mg lipopolysaccharide (LPS) per kg intravenously. LipoPGE1 was administered simultaneously at various concentrations (0.002. 0.02, 0.2, 2 mug/kg) in the tail vein. Blood samples and liver specimens were taken from the rats at 1, 3, 8. 12 and 24 h after injection with LPS alone or with LPS and Lipo PGE1. Serum macrophage inflammatory protein-2 (MIP-2) and cytokine-induced neutrophil chemoattractant (CINC) levels were measuredby the enzyme-linked immunosorbant assay using the corresponding antibodies. Liver specimens were fixed, and the number of neutrophils that had infiltrated each liver section was determined under a microscope. Serum alanine aminotransferase (ALT) levels were significantly lower in the rats injectedwith LPS and Lipo PGE1 compared with those in the rats injected with LPS alone, and this difference was expressed in a PGE1 dose-dependent manner. Serum MIP-2 levels were significantly lower at 3 h (141.4 +/- 95.5 pg/ml) and8 h (44.9 +/- 34.7 pg/ml) after injection with LPS and Lipo-PGE1 (2 mug/kg) than at the same times after injection with LPS alone (342.9 +/- 35.9 and358.3 +/- 23.4 pg:ml, respectively). Similarly, serum CINC levels were significantly lower at 8 h (482.7 +/- 156.0 ng/ml) after injection with LPS and Lipo-PGE1 (2 mug/kg) than at the same time after injection LPS alone (723.3 +/- 29.0 ng/ml). No significant differences were observed at any time between serum tumor necrosis factor-alpha (TNF-alpha) levels in rats injectedwith LPS alone and in rats injected with LPS and Lipo-PGE1 (2 mug/kg). Thenumber of neutrophils that had infiltrated the liver was significantly lower at 8 h after injection with LPS and Lipo PGE1 than at the same time after injection with LPS alone. This difference was expressed in a Lipo PGE1 dose-dependent manner. In conclusion, Lipo PGE1 reduces liver injury and serum levels of MIP-2 and CINC, bur not TNF-alpha, in rats injected with LPS and also reduces the number of neutrophils that infiltrate in the liver. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:35:10