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Titolo:
Real-time detection system for quantitation of hepatitis C virus RNA: a comparison with the other three methods
Autore:
Kishimoto, H; Yoshioka, K; Yano, M; Ukai, K; Ito, H; Watanabe, K; Kawamata, O; Kakumu, S;
Indirizzi:
Nagoya Univ, Sch Med, Dept Internal Med 3, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan SRL Inc, Ctr Mol Biol & Cytogenet, Tokyo, Japan SRL Inc Tokyo JapanSRL Inc, Ctr Mol Biol & Cytogenet, Tokyo, Japan Aichi Med Sch, Dept Internal Med 1, Aichi, Japan Aichi Med Sch Aichi Japan hi Med Sch, Dept Internal Med 1, Aichi, Japan
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 1, volume: 19, anno: 2001,
pagine: 12 - 21
SICI:
1386-6346(200101)19:1<12:RDSFQO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-B-HEPATITIS; RECOMBINANT INTERFERON-ALFA; NON-A; CONTROLLED TRIAL; PCR; THERAPY; SERUM; ASSAY; QUANTIFICATION; ANTIBODY;
Keywords:
genotype; viral load; interferon; amplicor HCV monitor test; amplicor HCV test; branched DNA assay; HCV core protein assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshioka, K Nagoya Univ, Sch Med, Dept Internal Med 3, Showa Ku, 65 Tsuruma Cho, Nagoya, Aichi 4668550, Japan Nagoya Univ 65 Tsuruma Cho Nagoya AichiJapan 4668550 0, Japan
Citazione:
H. Kishimoto et al., "Real-time detection system for quantitation of hepatitis C virus RNA: a comparison with the other three methods", HEPATOL RES, 19(1), 2001, pp. 12-21

Abstract

Real-time detection (RTD) system for quantitation of hepatitis C virus (I-ICV) was developed. Its sensitivity and usefulness were compared with the other three commercially available methods for quantitation of HCV. The seraof 166 patients positive for serum HCV RNA by Amplicor HCV test were assessed. HCV was detected in 78.5% (128/163) by branched DNA assay, in 88.8%(111/125) by HCV core protein assay, in 94.5% (156/165) by Amplicor HCV Monitor test, and in 97.0% (161/166) by the RTD system. The values of viral load by the RTD system were significantly well correlated with these obtained bythe other three methods. In the 50 patients treated by interferons (IFNs),the range which predicts the highest sustained response rate was less than0.5 Meq/ml for branched DNA assay (sustained response rate: 57.9%(11/19)),less than 1 kcopies/ml for Amplicor HCV Monitor test (85.7% (6/7)), and less than 10(4) copies/ml for RTD system (100% (7/7). None of the patients with greater than or equal to 2.8 Meq/ml by branched DNA assay (n = 14), greater than or equal to 250 kcopies/ml by Amplicor I-ICV Monitor test (n = 19), or greater than or equal to 2 x 10(6) copies/ml by RTD system (n = 16) obtained sustained response. In conclusion, RTD system was demonstrated to bethe most sensitive method for quantitation of HCV, and useful For the prediction of sustained response to IFN therapy. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:32:01