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Titolo:
The galU gene of Streptococcus pneumoniae that codes for a UDP-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies
Autore:
Mollerach, M; Garcia, E;
Indirizzi:
CSIC, Ctr Invest Biol, Dept Mol Microbiol, E-28006 Madrid, Spain CSIC Madrid Spain E-28006 iol, Dept Mol Microbiol, E-28006 Madrid, Spain Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol, RA-1113 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1113 Aires, DF, Argentina
Titolo Testata:
GENE
fascicolo: 1-2, volume: 260, anno: 2000,
pagine: 77 - 86
SICI:
0378-1119(200012)260:1-2<77:TGGOSP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPSULAR POLYSACCHARIDE; IDENTIFICATION; BIOSYNTHESIS; SEQUENCE; CLUSTER; RECOMBINATION; PNEUMOCOCCI; RESISTANCE; EVOLUTION; DIRECTS;
Keywords:
gene polymorphism; horizontal gene transfer; pneumococcus; UTP-glucose-1-phosphate uridylyltransferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, E CSIC, Ctr Invest Biol, Dept Mol Microbiol, Velazquez 144, E-28006 Madrid, Spain CSIC Velazquez 144 Madrid Spain E-28006 , E-28006 Madrid, Spain
Citazione:
M. Mollerach e E. Garcia, "The galU gene of Streptococcus pneumoniae that codes for a UDP-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies", GENE, 260(1-2), 2000, pp. 77-86

Abstract

The enzyme UTP-glucose-1-phosphate uridylyltransferase (UDP-glucose pyrophosphorylase, UDPG:PP) is synthesized by practically all organisms, althoughprokaryotic UDPG:PPs are evolutionarily unrelated to the eukaryotic counterparts. The primary structure of prokaryotic UDPG:PPs is well conserved, although little information exists on the polymorphism of the genes coding for these enzymes. It has been reported that the galU gene encoding the Streptococcus pneumoniae UDPG:PP is absolutely required for the synthesis of thecapsular polysaccharide, a sine qua non prerequisite for virulence. A 594 bp fragment covering 66% of the galU gene from 37 pneumococcal isolates andthe type strains of Streptococcus mitis, Streptococcus oralis, Streptococcus gordonii, Streptococcus sanguinis, Streptococcus salivarius, and Streptococcus sobrinus has been amplified by PCR and sequenced. Up to 21 differentalleles were found in S. pneumoniae. They possess a mosaic-like structure and belong to, at least, two evolutionarily distinct families that show a sequence divergence of 15-20%. In spite of its marked polymorphism, phylogenetic relationships among pneumococcal strains deduced from the galU gene matched those previously established by using alternative approaches. Comparison of the pneumococcal galU alleles with those from other streptococci indicated the existence of a complex network of genetic interchange. The galU gene represents an informative marker to be used alone or in conjunction with other molecular typing methods. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:33:46