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Titolo:
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity
Autore:
Sato, E; Koyama, S; Robbins, RA;
Indirizzi:
So Arizona Vet Hlth Care Syst, Res Serv, Tucson, AZ USA So Arizona Vet Hlth Care Syst Tucson AZ USA st, Res Serv, Tucson, AZ USA Univ Arizona, Dept Med, Tucson, AZ USA Univ Arizona Tucson AZ USAUniv Arizona, Dept Med, Tucson, AZ USA Shinshu Univ, Sch Med, Dept Internal Med 1, Matsumoto, Nagano 390, Japan Shinshu Univ Matsumoto Nagano Japan 390 d 1, Matsumoto, Nagano 390, Japan
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 5, volume: 16, anno: 2000,
pagine: 951 - 958
SICI:
0903-1936(200011)16:5<951:BSLFAE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY FIBROSIS; PLATELET-ACTIVATING-FACTOR; BRONCHOALVEOLAR LAVAGE; EXTRACELLULAR-MATRIX; IN-VITRO; EXPRESSION; ACCUMULATION; NEUTROPHIL; CYTOKINES; INJURY;
Keywords:
chemotaxis; eosinophil; eotaxin; granulocyte-macrophage colony-stimulating factor; leukotriene B-4; regulated on activation, normal T-cell; expressed and presumably secreted;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Robbins, RA Vet Adm Med Ctr, Res Hlth Care Grp, 3601 S 6th Ave, Tucson, AZ85723 USA Vet Adm Med Ctr 3601 S 6th Ave Tucson AZ USA 85723 Z 85723 USA
Citazione:
E. Sato et al., "Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity", EUR RESP J, 16(5), 2000, pp. 951-958

Abstract

The presence of eosinophils in the lungs of patients with pulmonary fibrosis correlates with poor prognosis or resistance to therapy. Furthermore, eosinophils localize to areas undergoing active fibrosis. It was hypothesizedthat a human lung fibroblast (HFL-1) and a human lung epithelial cell line(BEAS-2B) might release eosinophil chemotactic activity (ECA) in response to bleomycin, a chemotherapeutic agent associated with pulmonary fibrosis. HFL-1 and BEAS-2B cells were cultured in the presence of bleomycin and their supernatant fluids evaluated for ECA by means of a Boyden chamber method. HFL-1 and BEAS-2B cells released ECA in a dose- and time-dependent manner in response to bleomycin, and partial characterization revealed that the ECA was heterogeneous. ECA release from HFL-1 and BEAS-2B cells was significantly reduced by a leukotriene B-4 (LTB4) receptor antagonist and an antibody directed against granulocyte-macrophage colony-stimulating factor. HFL-1 cells released LTB4, eotaxin, and GM-CSF constitutively, and BEAS-2B cells released LTB4, eotaxin, regulated on activation, normal T-cell expressed and presumably secreted, and GM-CSF constitutively. In both cases, the release of GM-CSF was significantly increased in response to bleomycin. These data suggest that lung fibroblasts and epithelial cells may modulateeosinophil recruitment into the lung in bleomycin-induced pulmonary fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 11:20:48