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Titolo:
Electrical stimulation accelerates and increases expression of BDNF and trkB rnRNA in regenerating rat femoral motoneurons
Autore:
Al-Majed, AA; Brushart, TM; Gordon, T;
Indirizzi:
Univ Alberta, Dept Pharmacol, Div Neurosci, Edmonton, AB T6G 2S2, Canada Univ Alberta Edmonton AB Canada T6G 2S2 sci, Edmonton, AB T6G 2S2, Canada Johns Hopkins Univ, Sch Med, Dept Orthopaed Surg, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA pt Orthopaed Surg, Baltimore, MD USA Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Neurol, Baltimore, MD 21205 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 12, volume: 12, anno: 2000,
pagine: 4381 - 4390
SICI:
0953-816X(200012)12:12<4381:ESAAIE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; RETROGRADE AXONAL-TRANSPORT; FACTOR MESSENGER-RNA; NEUROTROPHIC FACTOR; MOTOR-NEURONS; HIPPOCAMPAL-NEURONS; PERIPHERAL-NERVE; GLUTAMATE RECEPTORS; SENSORY NEURONS; TROPHIC FACTOR;
Keywords:
in situ hybridization; intramuscular labelling; motor axonal regeneration; neurotrophin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Gordon, T Univ Alberta, Dept Pharmacol, Div Neurosci, 513 Heritage Med ResCtr, Edmonton, AB T6G 2S2, Canada Univ Alberta 513 Heritage Med Res Ctr Edmonton AB Canada T6G 2S2
Citazione:
A.A. Al-Majed et al., "Electrical stimulation accelerates and increases expression of BDNF and trkB rnRNA in regenerating rat femoral motoneurons", EUR J NEURO, 12(12), 2000, pp. 4381-4390

Abstract

Electrical stimulation promotes the speed and accuracy of motor axonal regeneration. The positive effects of stimulation are mediated at the cell body. Here we characterize the effect of electrical stimulation on motoneuronal expression of BDNF and its receptor, trkB, two genes whose expression levels in motoneurons correlate with regeneration and are regulated by electrical activity in a variety of neurons. We used semiquantitative in situ hybridization to measure expression of mRNA encoding BDNF and the full-length trkB receptor at intervals of 8 h, 2 days and 7 days after unilateral femoral nerve cut, suture, and stimulation. Expression in regenerating motoneurons was compared to that of contralateral intact motoneurons. BDNF and trkB signals were not significantly upregulated 8 h and 2 days after femoral nerve suture and sham stimulation. By 7 days, there was a 2-fold increase in both BDNF and trkB mRNA expression. In contrast, stimulation of cut and repaired nerves for only 1 h led to rapid upregulation of BDNF and trkB mRNA by 3-fold and 2-fold, respectively, within the first 8 h. The stimulation effect peaked at 2 days with 6-fold and 4-fold increases in the signals, respectively. Thereafter, the levels of BDNF and trkB mRNA expression declined toequal the 2-fold increase seen at 7 days after nerve repair and sham-stimulation. We conclude that brief electrical stimulation stimulates BDNF and trkB expression in regenerating motoneurons. Because electrical stimulation is known to accelerate axonal regeneration, we suggest that changes in the expression of BDNF and trkB correlate with acceleration of axonal regeneration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 21:56:49