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Titolo:
Recent advances in affinity capillary electrophoresis
Autore:
Guijn, RMGV; Frank, J; van Dedem, GWK; Baltussen, E;
Indirizzi:
Delft Univ Technol, Kluyver Lab Biotechnol, Dept Analyt Biotechnol, NL-2628 BC Delft, Netherlands Delft Univ Technol Delft Netherlands NL-2628 BC 28 BC Delft, Netherlands
Titolo Testata:
ELECTROPHORESIS
fascicolo: 18, volume: 21, anno: 2000,
pagine: 3905 - 3918
SICI:
0173-0835(200012)21:18<3905:RAIACE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONE-ELECTROPHORESIS; GEL-ELECTROPHORESIS; BINDING ISOTHERMS; FRONTAL ANALYSIS; PROTEINS; SEPARATION; PEPTIDES; DRUGS; DNA; HYDROPHOBICITY;
Keywords:
affinity capillary electrophoresis; binding constants; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Baltussen, E Delft Univ Technol, Kluyver Lab Biotechnol, Dept Analyt Biotechnol, Julianalaan 67, NL-2628 BC Delft, Netherlands Delft Univ Technol Julianalaan 67 Delft Netherlands NL-2628 BC
Citazione:
R.M.G.V. Guijn et al., "Recent advances in affinity capillary electrophoresis", ELECTROPHOR, 21(18), 2000, pp. 3905-3918

Abstract

Use of the specificity of (bio)interactions can effectively overcome the selectivity limitation faced in capillary electrophoresis (CE), and the resulting technique usually is referred to as affinity capillary electrophoresis (ACE). Despite the high selectivity of ACE, several important problems still need to be addressed. A major issue in all CE separations, including ACE, is the concentration detection limit. Using UV detection, this is usually in the order of 10(-6) M whereas laser-induced fluorescence (LIF) detection can provide detection limits down to the sub-10(-10) M range. However, amarked disadvantage of LIF is that labeling of the analytes is usually required, which might change the interaction behavior of the solutes under investigation. Additionally, labeling reactions at sub-10(-10) M concentrationlevels are certainly not trivial and often difficult to perform quantitatively. Alternative and universal detection approaches, particularly mass spectrometric (MS) detection, look very promising but (A) CE-MS techniques arestill far from routine application. important future progress in sensitivedetection strategies is likely to increase the use of AGE in the future.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:05:42