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Titolo:
Increased alveolar macrophage nuclear factor-kappa B activation and macrophage inhibitory protein-1 alpha levels in lung transplant patients
Autore:
Farver, CF; Raychardhuri, B; Malur, A; Drazba, J; Maurer, J; Tubbs, R; Mehta, AC; Schilz, R; Thomassen, MJ;
Indirizzi:
Cleveland Clin Fdn, Dept Anat Pathol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 Pathol, Cleveland, OH 44195 USA Cleveland Clin Fdn, Dept Pulm & Crit Care Med, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 re Med, Cleveland, OH 44195 USA Cleveland Clin Fdn, Dept Cell Biol, Cleveland, OH 44195 USA Cleveland ClinFdn Cleveland OH USA 44195 l Biol, Cleveland, OH 44195 USA Cleveland Clin Fdn, Dept Neurosci, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 urosci, Cleveland, OH 44195 USA Cleveland Clin Fdn, Transplantat Ctr, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 at Ctr, Cleveland, OH 44195 USA
Titolo Testata:
TRANSPLANTATION
fascicolo: 11, volume: 70, anno: 2000,
pagine: 1599 - 1603
SICI:
0041-1337(200012)70:11<1599:IAMNFB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; OBLITERATIVE BRONCHIOLITIS; CHRONIC REJECTION; FACTOR-BETA; RECIPIENTS; MIP-1-ALPHA; MANAGEMENT; ALLOGRAFTS; INFECTION; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Farver, CF Cleveland Clin Fdn, Dept Anat Pathol, Desk L25,9500 Euclid Ave,Cleveland,OH 44195 USA Cleveland Clin Fdn Desk L25,9500 Euclid Ave Cleveland OH USA 44195
Citazione:
C.F. Farver et al., "Increased alveolar macrophage nuclear factor-kappa B activation and macrophage inhibitory protein-1 alpha levels in lung transplant patients", TRANSPLANT, 70(11), 2000, pp. 1599-1603

Abstract

Background. Lung transplantation is increasingly used as the treatment formany end-stage pulmonary diseases. A major cause of morbidity and mortality in patients who undergo lung transplantation is rejection of the allograft, Proinflammatory macrophage-derived cytokines may sustain and/or enhance the immunological response to lung allograft antigens. Nuclear factor-kappaB (NF-kappaB) is a transcription factor that regulates the production of many of these cytokines and growth factors in alveolar macrophages (AMs). Theaim of our study was to evaluate the activation of NF-kappaB in AMs and the levels of one of the proinflammatory cytokines whose production it controls, macrophage inhibitory protein-1 alpha (MIP-1 alpha), in AMs from transplanted lungs compared to those from healthy controls. Methods. Twenty-eight (28) transplant recipients were included in the study. NF kappaB activation was evaluated by electrophoretic mobility shift assay of whole cell extracts and by immunohistochemical analysis on cytospin preparations. Concentrated bronchoalveolar lavage fluid was analyzed by enzyme-linked immunosorbent assay for MIP-1 alpha levels. Results. NF-kappaB was activated in alveolar macrophages from transplant patients as compared to healthy controls. MIP-1 alpha levels in epithelial-lining fluid were elevated in transplant patients as compared to healthy controls. Increased MIP-1 alpha levels correlated with viral infections in thetransplant patients. Neither finding was found to correlate with acute rejection by transbronchial biopsy. Conclusions. These results demonstrate that NF-kappaB activation and MIP-1alpha levels are increased in transplanted lungs and may play a role in the inflammatory cytokine cascade that leads to the long-term tissue damage and allograft rejection in these patients.

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Documento generato il 10/07/20 alle ore 17:41:13