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Titolo:
The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p
Autore:
Tan, W; Zolotukhin, AS; Bear, J; Patenaude, DJ; Felber, BK;
Indirizzi:
NCI, Basic Res Lab, Human Retrovirus Pathogenesis Sect, Frederick Canc Res& Dev Ctr, Frederick, MD 21702 USA NCI Frederick MD USA 21702 ick Canc Res& Dev Ctr, Frederick, MD 21702 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 12, volume: 6, anno: 2000,
pagine: 1762 - 1772
SICI:
1355-8382(200012)6:12<1762:TMEICE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; MESSENGER-RNA EXPORT; CONSTITUTIVE TRANSPORT ELEMENT; DOUBLE-STRANDED-RNA; NUCLEAR EXPORT; HUMAN TAP; NUCLEOCYTOPLASMIC TRANSPORT; GENETIC INTERFERENCE; REV; PROTEIN;
Keywords:
import; nuclear rim; nucleocytoplasmic transport; nucleoporin; RNA binding; RNA interference; shuttling protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Felber, BK NCI, Basic Res Lab, Human Retrovirus Pathogenesis Sect, Frederick Canc Res& Dev Ctr, Bldg 535,Room 110, Frederick, MD 21702 USA NCI Bldg 535,Room 110 Frederick MD USA 21702 rick, MD 21702 USA
Citazione:
W. Tan et al., "The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p", RNA, 6(12), 2000, pp. 1762-1772

Abstract

Human TAP and Saccharomyces cerevisiae Mex67p belong to a family of proteins that mediate mRNA export. Computer searches identified previously two Caenorhabditis elegans genes, C15H11.3 and C15H11.6, that encode putative homologs of hTAP and Mex67p (Segref et al., EMBO J, 1997, 16:3256-3271). UsingRNA interference experiments in C. elegans, we found that functional knockout of C15H11.3 resulted in nuclear accumulation of poly(A)-containing RNAsand was lethal for both embryos and adult nematodes. No embryonic or progeny abnormality was observed in functional knockout of C15H11.6. Taken together, these data established that the C15H11.3 gene product is an ortholog of hTAP and Mex67p; thus, it was named Ce-NXF-1. Ce-NXF-1 binds RNA directlyand is a nucleocytoplasmic shuttle protein accumulating in the nucleoplasmand at the nuclear rim. The rim association is mediated via unique signalspresent in the C-terminal portion of all TAP/NXF and Mex67p proteins. Thisregion was shown to interact with the PC-repeat domains of nucleoporins Nup98, Nup153, and Nup214, indicating that the rim association occurs throughcomponents of the nuclear pore complex. In summary, Ce-NXF-1 belongs together with hTAP and Mex67p to a family of proteins that participate in mRNA export and can provide a direct molecular link between mRNAs and components of the nuclear pore complex. Therefore, despite differences in mRNA metabolism between these species, they utilize a conserved mRNA transport mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 04:58:53