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Titolo:
Minor structural consequences of alternative CUG codon usage (Ser for Leu)in Candida albicans exoglucanase
Autore:
Cutfield, JF; Sullivan, PA; Cutfield, SM;
Indirizzi:
Univ Otago, Sch Med Sci, Dept Biochem, Dunedin, New Zealand Univ Otago Dunedin New Zealand Sci, Dept Biochem, Dunedin, New Zealand
Titolo Testata:
PROTEIN ENGINEERING
fascicolo: 10, volume: 13, anno: 2000,
pagine: 735 - 738
SICI:
0269-2139(200010)13:10<735:MSCOAC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-CODE; EXO-BETA-(1,3)-GLUCANASE; HYDROLASES; MOLSCRIPT;
Keywords:
alternative codon usage; Candida albicans; crystal structure; exoglucanase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Cutfield, JF Univ Otago, Sch Med Sci, Dept Biochem, POB 56, Dunedin, New Zealand Univ Otago POB 56 Dunedin New Zealand Dunedin, New Zealand
Citazione:
J.F. Cutfield et al., "Minor structural consequences of alternative CUG codon usage (Ser for Leu)in Candida albicans exoglucanase", PROTEIN ENG, 13(10), 2000, pp. 735-738

Abstract

In some species of Candida the CUG codon is encoded as serine and not leucine, In the case of the exo-beta -1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64, Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64, Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encodedserines are also found at non-essential surface sites. However such codon usage is rare in C,albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:00:50