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Titolo:
A Rab1 GTPase is required for transport between the endoplasmic reticulum and Golgi apparatus and for normal Golgi movement in plants
Autore:
Batoko, H; Zheng, HQ; Hawes, C; Moore, I;
Indirizzi:
Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England Univ Oxford Oxford England OX1 3RB pt Plant Sci, Oxford OX1 3RB, England Oxford Brookes Univ, Res Sch Biol & Mol Sci, Oxford OX3 0BP, England Oxford Brookes Univ Oxford England OX3 0BP Sci, Oxford OX3 0BP, England
Titolo Testata:
PLANT CELL
fascicolo: 11, volume: 12, anno: 2000,
pagine: 2201 - 2217
SICI:
1040-4651(200011)12:11<2201:ARGIRF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; BINDING PROTEIN; NUCLEOTIDE EXCHANGE; VESICULAR TRANSPORT; SECRETORY PATHWAY; SUBCELLULAR-LOCALIZATION; GENE-EXPRESSION; COATED VESICLES; LIVING CELLS; BREFELDIN-A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Moore, I Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England Univ OxfordOxford England OX1 3RB Sci, Oxford OX1 3RB, England
Citazione:
H. Batoko et al., "A Rab1 GTPase is required for transport between the endoplasmic reticulum and Golgi apparatus and for normal Golgi movement in plants", PL CELL, 12(11), 2000, pp. 2201-2217

Abstract

We describe a green fluorescent protein (GFP)-based assay for investigating membrane traffic on the secretory pathway in plants. Expression of AtRab1b(N121I), predicted to be a dominant inhibitory mutant of the Arabidopsis Rab GTPase AtRab1b, resulted in accumulation of a secreted GFP marker in an intracellular reticulate compartment reminiscent of the endoplasmic reticulum. This accumulation was alleviated by coexpressing wild-type AtRab1b but not AtRab8c. When a Golgi-targeted and N-glycosylated variant of GFP was coexpressed with AtRab1b(N121I), the variant also accumulated in a reticulatenetwork and an endoglycosidase H-sensitive population appeared. Unexpectedly, expression of AtRab1b(N121I), but not of the wild-type AtRab1b, resulted in a reduction or cessation of vectorial Golgi movement, an effect that was reversed by coexpression of the wild type. We conclude that AtRab1b function is required for transport from the endoplasmic reticulum to the Golgi apparatus and suggest that this process may be coupled to the control of Golgi movement.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:06:14