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Titolo:
Mineralocorticoid hormone signaling regulates the 'epithelial sodium channel' in fibroblasts from human cornea
Autore:
Mirshahi, M; Mirshahi, S; Golestaneh, N; Nicolas, C; Mishal, Z; Lounes, KC; Hecquet, C; Dagonet, F; Pouliquen, Y; Agarwal, MK;
Indirizzi:
INSERM, XR86, F-75270 Paris 06, France INSERM Paris France 06INSERM, XR86, F-75270 Paris 06, France CNRS, Ctr Cordeliers, Paris, France CNRS Paris FranceCNRS, Ctr Cordeliers, Paris, France INSERM, U9912, Paris, France INSERM Paris FranceINSERM, U9912, Paris, France CNRS, UMR 8532, Dept Biol & Pharmacol Struct, Villejuif, France CNRS Villejuif France , Dept Biol & Pharmacol Struct, Villejuif, France CNRS, IFC 122, Lab Cytometries, Villejuif, France CNRS Villejuif FranceCNRS, IFC 122, Lab Cytometries, Villejuif, France
Titolo Testata:
OPHTHALMIC RESEARCH
fascicolo: 1, volume: 33, anno: 2001,
pagine: 7 - 19
SICI:
0030-3747(200101/02)33:1<7:MHSRT'>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINAL-PIGMENT EPITHELIUM; COLLECTING DUCT; CATION CHANNEL; NA+ CHANNEL; RECEPTOR; GENE; CELLS; PHOSPHORYLATION; NA+,K+-ATPASE; EXPRESSION;
Keywords:
cornea; fibroblasts; aldosterone; mineralocorticoid receptor; sodium channel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Mirshahi, M INSERM, XR86, 15 Rue Ecole Med, F-75270 Paris 06, France INSERM 15 Rue Ecole Med Paris France 06 5270 Paris 06, France
Citazione:
M. Mirshahi et al., "Mineralocorticoid hormone signaling regulates the 'epithelial sodium channel' in fibroblasts from human cornea", OPHTHAL RES, 33(1), 2001, pp. 7-19

Abstract

We investigated the regulation of sodium absorption by steroid hormones inembryologically diverse cells from the human eye. A cell extract from human corneal fibroblasts was positive for both the epithelial sodium channel (ENaC) and the mineralocorticoid receptor (MCR) as 82- to 85-kD and 102-kD bands, respectively, by the Western blot technique. In fluorescent, confocaland electron microscopy, the MCR was revealed as a nucleocytoplasmic protein, whereas the ENaC was almost exclusively membrane bound; both appeared aligned along actin filaments of corneal keratocytes, and both were widely colocalized in various cell types of human cornea in situ. Following reversetranscription and amplification of total RNA isolated from corneal fibroblasts, the ENaC and MCR genes in the PCR product were evident as predicted bands of 520 and 843 bp, respectively, whose sequence exhibited 100% identity with those from known human sources. The multiplication of corneal fibroblasts was influenced by both the MCR-specific antagonist RU 26752 and the natural hormone aldosterone, and these steroids also stimulated protein phosphorylation. In quantitative PCR, both the basal and aldosterone-induced levels of ENaC were diminished by the MCR-specific antagonist ZK 91587. Consequently, the ocular sodium channel appears to be regulated by steroid signalling in cells of diverse embryological origins, contrary to the existing notions where (a) this process would be limited exclusively to the epithelial cells and (b) ocular sodium transport would be regulated via the Na+-K+-ATPase in the basolateral membrane. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:23:57