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Titolo:
A receptor-specific peptide for imaging infection and inflammation
Autore:
Rao, PS; Pallela, VR; Vassileva-Belnikolovska, D; Jungkind, D; Thakur, ML;
Indirizzi:
Thomas Jefferson Univ Hosp, Philadelphia, PA 19107 USA Thomas Jefferson Univ Hosp Philadelphia PA USA 19107 elphia, PA 19107 USA
Titolo Testata:
NUCLEAR MEDICINE COMMUNICATIONS
fascicolo: 11, volume: 21, anno: 2000,
pagine: 1063 - 1070
SICI:
0143-3636(200011)21:11<1063:ARPFII>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOTACTIC FACTORS; BLOOD-CELLS; ANTI-SSEA-1; NEUTROPENIA; LEUKOCYTES; ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Thakur, ML Thomas Jefferson Univ, 1020 Locust St,Suite 359 JAH, Philadelphia, PA 19107 USA Thomas Jefferson Univ 1020 Locust St,Suite 359 JAH Philadelphia PA USA 19107
Citazione:
P.S. Rao et al., "A receptor-specific peptide for imaging infection and inflammation", NUCL MED C, 21(11), 2000, pp. 1063-1070

Abstract

The chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMLP), when radiolabelled, continues to be an attractive agent for imaging infection or inflammation. Previously, several analogues of fMLP have been prepared and radiolabelled using a bifunctional chelating agent conjugation procedurethat was relatively long and complex. We have prepared a new analogue of fMLP, TP765, by the addition of 4-aminobutyric acid (4-ABA) and a group of four amino acids, Gly-Gly-d-Ala-Gly, to the carboxy terminus (i.e. to the phenylalanine) of fMLP. The adduct -(4-ABA)-Gly-Gly-d-Ala-Gly- serves as a chelating moiety for strong chelation with Tc-99(m). The use of a peptide as a chelating moiety greatly simplified the synthetic procedure and rendered the analogue ready for instant chelation with Tc-99(m). HPLC analysis revealed that Tc-99(m)-TP765 was a single chemical entity that retained biological activity and neutrophil specificity. Tc-99(m)-TP765 was stable when challenged with strong chelating agents in vitro and had rapid but biphasic blood clearance (alphat(1/2), = 7 min, betat(1/2) = 45 min). Approximately 90%of the radioactivity had cleared from circulation within 45 min post-injection and the agent had accumulated in experimental bacterial or sterile abscesses in significantly (P<0.05) higher quantities than the analogues evaluated previously. Generally, the biodistribution pattern of Tc-99(m)-TP765 was similar to that of other analogues examined and its abscess uptake was independent of the abscess age. In conclusion, a new analogue of fMLP, Tc-99(m)-TP765, was prepared by a simple procedure. This new analogue has properties similar to those of previously examined analogues used as agents for imaging infection or inflammation. ((C) 2000 Lippincott Williams & Wilkins).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:30:42