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Titolo:
Molecular characterisation of Heterorhabditis indica isolates from India, Kenya, Indonesia and Cuba
Autore:
Stack, CM; Easwaramoorthy, SG; Metha, UK; Downes, MJ; Griffin, CT; Burnell, AM;
Indirizzi:
Natl Univ Ireland, Dept Biol, Inst Bioengn & Agroecol, Maynooth, Kildare, Ireland Natl Univ Ireland Maynooth Kildare Ireland l, Maynooth, Kildare, Ireland Indian Council Agr Res, Sugarcane Breeding Inst, Coimbatore 641007, Tamil Nadu, India Indian Council Agr Res Coimbatore Tamil Nadu India 641007 mil Nadu, India
Titolo Testata:
NEMATOLOGY
, volume: 2, anno: 2000,
parte:, 5
pagine: 477 - 487
SICI:
1388-5545(2000)2:<477:MCOHII>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTOMOPATHOGENIC NEMATODES STEINERNEMA; N-SP RHABDITIDA; INTERGENIC SPACER; HYBRID DYSGENESIS; RIBOSOMAL DNA; IDENTIFICATION; DROSOPHILA; HELIOTHIDIS; POPULATION; EVOLUTION;
Keywords:
crossbreeding; DNA probe; entomopathogenic nematode; molecular diagnostics; rDNA ITS; rDNA IGS; satellite DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Burnell, AM Natl Univ Ireland, Dept Biol, Inst Bioengn & Agroecol, Maynooth, Kildare, Ireland Natl Univ Ireland Maynooth Kildare Ireland Kildare, Ireland
Citazione:
C.M. Stack et al., "Molecular characterisation of Heterorhabditis indica isolates from India, Kenya, Indonesia and Cuba", NEMATOLOGY, 2, 2000, pp. 477-487

Abstract

Isolates of Heterorhabditis were identified as H. indica using the following molecular diagnostic features: hybridisation to a H. indica specific satellite DNA probe; AluI and MboI restriction profiles of the rDNA ITS PCR product and the AluI profile of the rDNA IGS PCR product. The Kenyan isolatesrepresent a distinct subgroup of H. indica. These isolates lacked one of the two HinfI restriction sites which are present in the rDNA ITS product ofall the other isolates tested and they also differed from other H. indica isolates in their rDNA IGS HaeIII restriction profile. The Indian isolates are interfertile. The Kenyan isolates are interfertile but only one Kenyan isolate, Ki3, produced viable progeny when crossed with H. indica LN2. The four Indonesian isolates are interfertile, but only one Indonesian isolate (INA H1) produced viable hybrids when crossed with N. indica LN2. INA H1 was also interfertile with the Kenyan isolate Ki3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:11:14