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Titolo:
The Escherichia coli lacZ reversion mutagenicity assay
Autore:
Josephy, PD;
Indirizzi:
Univ Guelph, Guelph Waterloo Ctr Grad Work Chem & Biochem, Dept Chem & Biochem, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 Biochem, Guelph, ON N1G 2W1, Canada
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 455, anno: 2000,
pagine: 71 - 80
SICI:
1386-1964(20001120)455:1-2<71:TECLRM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CYTOCHROME-P450 1A2; TYPHIMURIUM TESTER STRAINS; ALLOW RAPID DETECTION; SALMONELLA-TYPHIMURIUM; AROMATIC-AMINES; MUTATIONAL SPECIFICITY; FRAMESHIFT MUTATIONS; TEST SYSTEM; SUPEROXIDE-DISMUTASE; CHEMICAL MUTAGENESIS;
Keywords:
lacZ; beta-galactosidase; E. coli; heterocyclic amines; aromatic amines; mutagenesis; P450 1A2; UvrABC; umuDC; arylamine N-acetyltransferase;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Josephy, PD Univ Guelph, Guelph Waterloo Ctr Grad Work Chem & Biochem, Dept Chem & Biochem, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 elph, ON N1G 2W1, Canada
Citazione:
P.D. Josephy, "The Escherichia coli lacZ reversion mutagenicity assay", MUT RES-F M, 455(1-2), 2000, pp. 71-80

Abstract

The Escherichia coli lacZ reversion assay, based on the set of episomal lacZ alleles engineered by Miller et al., provides an attractive system for studies of mutagenesis and mutational specificity. Each strain in the lacZ set reverts by a specific base substitution or frameshift event. Revertants are selected by growth on lactose minimal medium. In this review, I describe the development of the assay and its subsequent modifications and improvements. Examples of its application are presented and detailed protocols forthe implementation of the assay are given. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:04:19