Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PkwA, a WD-repeat protein, is expressed in spore-derived mycelium of Thermomonospora curvata and phosphorylation of its WD domain could act as a molecular switch
Autore:
Joshi, B; Janda, L; Stoytcheva, Z; Tichy, P;
Indirizzi:
Acad Sci Czech Republ, Inst Microbiol, Lab Physiol & Genet Actinomycetes, CR-14220 Prague 4, Czech Republic Acad Sci Czech Republ Prague Czech Republic 4 0 Prague 4, Czech Republic
Titolo Testata:
MICROBIOLOGY-UK
, volume: 146, anno: 2000,
parte:, 12
pagine: 3259 - 3267
SICI:
1350-0872(200012)146:<3259:PAWPIE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN KINASE-A; BETA-SUBUNIT; STREPTOMYCES GRANATICOLOR; SER/THR KINASE; COILED-COIL; GENE; IDENTIFICATION; DICTYOSTELIUM; CLONING; BACTERIUM;
Keywords:
PkwA; WD-repeat protein; protein Ser/Thr kinase; phosphorylation; Thermomonospora curvata;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Tichy, P Acad Sci Czech Republ, Inst Microbiol, Lab Physiol & Genet Actinomycetes, Videnska 1083, CR-14220 Prague 4, Czech Republic Acad Sci Czech Republ Videnska 1083 Prague Czech Republic 4 blic
Citazione:
B. Joshi et al., "PkwA, a WD-repeat protein, is expressed in spore-derived mycelium of Thermomonospora curvata and phosphorylation of its WD domain could act as a molecular switch", MICROBIO-UK, 146, 2000, pp. 3259-3267

Abstract

WD-repeat proteins are found in all eukaryotes and are implicated in a variety of regulatory functions as a result of protein-protein interactions. PkwA from Thermomonospora curvata CCM3352 is a first potential example of a WD-repeat protein in a prokaryotic actinomycete. A mAb (3G2) was generated against the carboxy terminus of PkwA and wa used to analyse the expression of PkwA in T. curvata. PkwA was detected in exponential growth phase following inoculation with spores, but could not be found at any stage of growth following inoculation with vegetative mycelium. PkwA and its WD domain wereexpressed in Escherichia coli as His-tag derivatives and purified on a Talon metal affinity matrix. The WD domain was phosphorylated by Pkg2, a membrane-spanning protein Ser/Thr kinase from 'Streptomyces granaticolor'. A membrane fraction from an exponential, spore-derived culture of T. curvata wasfound to phosphorylate the WD domain specifically in the presence of Mn2+. These data confirm that PkwA is expressed in spore-derived exponential growth phase of T. curvata and could play a role as a molecular switch in a signalling pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:05:30